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dc.contributor.authorSilva, Aldir Carlos
dc.date.accessioned2023-12-21T18:56:11Z-
dc.date.available2023-12-21T18:56:11Z-
dc.date.issued2013-12-19
dc.identifier.citationSILVA, Aldir Carlos. Efeito da fonte de nitrogênio na liberação de OH-/H+ na rizosfera e a interação com toxidez de alumínio, estresse de salinidade e associação com Trichoderma sp. 2013. 130 f. Tese (Programa de Pós-Graduação em Fitotecnia) - Universidade Federal Rural do Rio de Janeiro, Seropédica.por
dc.identifier.urihttps://rima.ufrrj.br/jspui/handle/20.500.14407/10033-
dc.description.abstractO presente trabalho foi realizado com o objetivo de estudar se variações do pH da rizosfera e do meio de crescimento, controladas pelo uso de fontes nitrogenadas, poderiam amenizar a toxidez de alumínio ou os efeitos provocados pela salinização. Na literatura cientifica é bem documentado que se uma planta esta absorvendo e assimilando nitrato como uma fonte nitrogenada, ela libera 0H- para o substrato de crescimento. Se ela esta absorvendo e assimilando uma fonte amoniacal libera H+. Isto ocorre devido às células necessitarem equilibrar o seu balanço eletroquímico de carga, devido à assimilação diferenciada de cátions e anions. Apesar de ser uma regra geral, os trabalhos científicos usam a fonte nitrogenada diretamente no meio de crescimento, poucos utilizaram outros métodos de adicionar estas fontes, como por exemplo, a aplicação foliar de nitrogênio e suas implicações no balanço de carga. Neste trabalho foram estudadas diversas alternativas de aplicação de nitrogênio e as suas interações com a toxidez provocada por alumínio, excesso de sais e com o fungo Trichoderma. Foram estudas diversas plantas: Girassol (Helianthus annuus L), Maracujá (Passiflora edulis f. flavicarpa L.), Abacaxi (Ananas comosus Merril), Café (Coffea arabica L), Amendoeira (Terminalia catapa Linn) e Sombreiro (Clitoria fairchildiana Howard). Para realizar os estudos com plantas de Girassol foi realizada inicialmente uma seleção de plantas mais tolerantes a acidez e a salinidade. Os experimentos foram realizados em diversos substratos, solo, areia, solução nutritiva simples e completa. A interação entre fungo Trichoderma x fonte nitrogenada foi realizado para plantas de Girassol e Maracujá. Foram selecionadas como medianamente tolerantes ao alumínio a cultivar de Girassol Hélio 360, as demais cultivares a toxidez ocorreu em contração igual ou superiora 160μM de alumínio. Foi selecionada como tolerante a salinidade as cultivares Hélio 251>250>253, com concentrações acima 25mM de NaCl (1,90 dS.m¹) as plantas não cresceram.Após esta etapa foram implantados experimentos com as cultivares previamente selecionas como sensíveis ou tolerantes e estas foram utilizadas de acordo com a necessidade experimental. Os experimentos foram divididos nas seguintes etapas: Na primeira etapa, avaliou-se a liberação do OH-/H+ com aplicação das fontes nitrogenadas diretamente no meio de crescimento, sendo verificado que as fontes nitrogenadas não conseguiram minimizar os efeitos tóxicos do alumínio e da salinidade. Na segunda etapa, avaliou-se os efeitos liberação do OH-/H+ com aplicação das fontes nitrogenadas na associação do fungo Trichoderma. Este fungo cresceu em placas de petri somente em valores de pH acima de 5,0. A liberação de OH-/H+ não influenciou a associação do fungo com as raízes de plantas de Girassol e Maracujá. Na terceira etapa, foi avaliado se a aplicação foliar das fontes nitrogenadas poderia produzir os mesmos os efeitos nos efluxos de cargas na rizosfera. Foi verificado que as fontes nitrogenadas aplicadas via foliar aumentaram o pH da solução quando foi aplicado 10% de nitrato e reduziram o pH quando foi aplicado 5% e 10% de sulfato de amônia em plantas de Café e com 10% de nitrato em Girassol. A aplicação foliar de fontes nitrogenadas não alteraram a toxidez de alumínio e nem do efeito salino em plantas de Café e Girassol.por
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico - CNPqpor
dc.formatapplication/pdf*
dc.languageporpor
dc.publisherUniversidade Federal Rural do Rio de Janeiropor
dc.rightsAcesso Abertopor
dc.subjectnitrogen sourceeng
dc.subjectaluminumeng
dc.subjectsalinityeng
dc.subjectTrichodermaeng
dc.subjectfontes de nitrogênio, ,por
dc.subjectalumíniopor
dc.subjectsalinidadepor
dc.subjectTrichodermapor
dc.titleEfeito da fonte de nitrogênio na liberação de OH-/H+ na rizosfera e a interação com toxidez de alumínio, estresse de salinidade e associação com Trichoderma sp.por
dc.title.alternativeEffect of nitrogen supply in release of OH-/ H+in the rhizosphere and interaction with aluminum toxicity, salinity stress and association with Trichoderma sppor
dc.typeTesepor
dc.description.abstractOtherThe present study was conducted to evaluate if changes in rhizosphere pH of the growth media, controlled by the use of nitrogen sources could alleviate aluminum toxicity or effects caused by salinization. It is well documented that if a plant is absorbing and assimilating nitrate as a nitrogen source, it releases 0H - for growth substrate. If she is absorbing, assimilating, ammonia source releases H+. This occurs because the cells need to balance its electrochemical charge balance due to differential uptake of cations and anions. Despite being a general rule, scientific research directly use the nitrogen source in the growth medium, few have used other methods to add these sources, such as foliar application of nitrogen and its implications on load balancing. In this work, several alternative management of nitrogen application and its interactions with the toxicity caused by aluminum, excess salts and Trichoderma were studied. Sunflower (Helianthus annuus L.), Passionflower (Passiflora edulis f flavicarpa L.), Pineapple (Ananas comosus Merril), Coffee (Coffea arabica L), Almond (Terminalia catapa Linn) and Sombrero (Clitoria fairchildiana Howard). To conduct studies with plants Sunflower was initially performed a selection of more tolerant to acidity and salinity plants. The experiments were conducted on various substrates, soil and sand, simple and complete nutrient solution. The interaction between Trichoderma x nitrogen source for plants was conducted with Passion fruit and Sunflower. Were selected as moderately tolerant to aluminum to grow Sunflower Helium 360 , the other cultivars to toxicity occurred in contraction equal to or superior 160μM aluminum . Was selected as tolerant to salinity the Hélio 251 > 250 > 253, cultivars, with concentrations above 25 mM NaCl (1.90 dS.m¹) plants were stop grown. After this step prior experiments cultivars tolerant or sensitive were used in accordance with the experimental needs. The experiments were divided into the following steps: In the first step, we evaluated the release of OH - / H + with application of nitrogen sources directly in the growth medium, and found that the nitrogen sources were unable to minimize the toxic effects of aluminum and salinity. In the second experiment, the effects of the release OH- /H+ with application of nitrogen sources in the association of the fungus Trichoderma sp. This fungus grown in petri dishes only at pH values above 5.0. The release of OH- / H+ did not influence the association of the fungus with the roots of plants of sunflower and Passion fruit. In the third step, we assessed whether foliar application of nitrogen sources could produce the same effects on efflux of loads in the rhizosphere. It was found that the nitrogen sources applied foliar increased the pH of the solution was applied when 10 % of nitrate and reduced the pH when applied 5 % and 10 % ammonium sulfate in plants of Coffee and 10 % of nitrate in Sunflower . The foliar application of nitrogen sources did not alter the toxicity of aluminum and not the salt effect on plants and Sunflower Coffee.eng
dc.contributor.advisor1Jacob Neto, Jorge
dc.contributor.advisor1ID8850585187por
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/6508017274417976por
dc.contributor.referee1Zonta, Everaldo
dc.contributor.referee2Goi, Silvia Regina
dc.contributor.referee3Silva, Eliane Maria Ribeiro da
dc.contributor.referee4Baldani, Vera Lucia D.
dc.creator.ID4166070711por
dc.creator.Latteshttp://lattes.cnpq.br/6832034287534111por
dc.publisher.countryBrasilpor
dc.publisher.departmentInstituto de Agronomiapor
dc.publisher.initialsUFRRJpor
dc.publisher.programPrograma de Pós-Graduação em Fitotecniapor
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