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dc.contributor.authorLima, Nathanielly Rocha Casado de
dc.date.accessioned2023-12-22T01:42:54Z-
dc.date.available2023-12-22T01:42:54Z-
dc.date.issued2011-03-30
dc.identifier.citationLIMA, Nathanielly Rocha Casado de. Análise do crescimento, diferenciação celular e perfil isoenzimático de um isolado de tripanosomatídeo obtido de Megaselia scalaris Loew, 1866 (Díptera, Phoridae). 2011. 54 f. Dissertação (Mestrado em Biologia Animal) - Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2011.por
dc.identifier.urihttps://rima.ufrrj.br/jspui/handle/20.500.14407/10768-
dc.description.abstractA família Trypanosomatidae inclui parasitas de uma grande variedade de vertebrados, invertebrados (principalmente insetos), plantas e algumas espécies de protozoários. No presente trabalho, um isolado foi obtido por coprocultivo de trato intestinal de Megaselia scalaris Loew, 1866 (Diptera, Phoridae). O isolado original e seus clones (obtidos por citometria de fluxo) foram depositados na "Coleção de Tripanosomatídeos do Instituto Oswaldo Cruz". Estes clones foram caracterizados por diversas abordagens em comparação com espécies de referência de diferentes gêneros. Seu crescimento foi estudado em meio LIT (com 10 ou 20% de soro fetal bovino) modificado, de acordo com o nível de exigência. O número de células por microlitro foi estimado em intervalos de 24 h, entre 48-144 h. Esfregaços corados pelo Giemsa (após hidrólise ácida) foram preparados visando estudos de diferenciação celular e morfometria das formas encontradas. A análise de isoenzimas foi realizada utilizando-se os seguintes sistemas: GPI , PGM, 6PGDH, ME, IDH, MDH, ACON, FUM, MPI e HK. Os dados desta análise foram processados numericamente e submetidos à análise computacional utilizando-se o coeficiente de Jaccard e o algoritmo UPGMA. O conjunto de resultados deste trabalho sugere que: (1) O isolado de M. scalaris apresentou similaridade de 70% com Herptomonas megaseliae; (2) Esse isolado revelou a presença exclusiva de promastigotas em toda análise da curva de crescimento, como esperado para o gênero Leptomonas, entretanto as formas exibiram grande polimorfismo. Estudos complementares utilizando ferramentas moleculares serão realizados.por
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESpor
dc.formatapplication/pdf*
dc.languageporpor
dc.publisherUniversidade Federal Rural do Rio de Janeiropor
dc.rightsAcesso Abertopor
dc.subjectTripanosomatídeos inferiorespor
dc.subjectmorfobiologia e isoenzimaspor
dc.titleAnálise do crescimento, diferenciação celular e perfil isoenzimático de um isolado de tripanosomatídeo obtido de Megaselia scalaris Loew, 1866 (Díptera, Phoridae)por
dc.title.alternativeAnalysis of growth, cell differentiation and isoenzyme profile of an isolated trypanosomatid obtained Megaselia scalaris Loew, 1866 (Diptera, Phoridae)eng
dc.typeDissertaçãopor
dc.description.abstractOtherThe Trypanosomatidae family includes parasites of a wide variety of vertebrates, invertebrates (mainly insects), plants and some species of protozoa. In this study, an isolate was obtained by feces culturing of the intestinal tract of Megaselia scalaris Loew, 1866 (Diptera, Phoridae). The original isolate and its clones (obtained by flow cytometry) were deposited in the ―Trypanosomatids Collection of the Instituto Oswaldo Cruz. These clones were characterized by different approaches I in comparison with the reference species of different genera. Its growth has been studied in LIT medium (with 10 or 20% fetal bovine serum) modified according to the requirement. The number of cells per microliter was estimated at 24 h intervals between 48-144 h. Giemsa-stained smears (after acid hydrolysis) were prepared in order to study cell differentiation and morphology of the forms. The isozyme analysis was performed using the following systems: GPI, PGM, 6PGDH, ME, IDH, MDH, ACON, FUM, MPI and HK. Data from this analysis were processed and analyzed numerically using the computacional Jaccard coefficient and UPGMA algorighm. The result set of this study suggest that: (1) The strain of M. scalaris showed 70% similarity with Herpetomonas megaseliae, (2) This isolate showed the exclusive presence of promastigotes in any analysis of the growth curve as expected for the genus Leptomonas, however the forms exhibited high polymorphism. Additional studies using molecular tools will be realizedeng
dc.contributor.advisor1Mallet, Jacenir Reis dos Santos
dc.contributor.advisor1ID710.008.957-34por
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/9643185827631520por
dc.contributor.referee1Gomes, Suzete Araújo Oliveira
dc.contributor.referee2Gonçalves, Teresa Cristina Monte
dc.creator.ID8207359730por
dc.creator.Latteshttp://lattes.cnpq.br/3757620013031888por
dc.publisher.countryBrasilpor
dc.publisher.departmentInstituto de Ciências Biológicas e da Saúdepor
dc.publisher.initialsUFRRJpor
dc.publisher.programPrograma de Pós-Graduação em Biologia Animalpor
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dc.subject.cnpqParasitologiapor
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dc.originais.urihttps://tede.ufrrj.br/jspui/handle/jspui/1224
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