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dc.contributor.authorSouza, Raquel do Nascimento de
dc.date.accessioned2023-12-21T19:00:42Z-
dc.date.available2023-12-21T19:00:42Z-
dc.date.issued2017-05-17
dc.identifier.citationSOUZA, Raquel do Nascimento de. A apipuntura influencia o balanço de fenótipos de micróglia/macrófagos, a neuroinflamação e o fator anti-apoptótico no modelo de lesão medular por compressão em ratos wistar. 2017. 91 f. Tese (Doutorado Multicêntrico em Ciências Fisiológicas) - Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Seropédica - RJ, 2017.por
dc.identifier.urihttps://rima.ufrrj.br/jspui/handle/20.500.14407/10302-
dc.description.abstractO objetivo do presente estudo foi investigar os tipos celulares e os mecanismos fisiológicos envolvidos com a apipuntura no modelo de lesão medular moderada (SCI) por compressão. Para indução da SCI, um cateter fogarty de embolectomia foi inflado com 15μL de água destilada no espaço extradural, entre as vértebras T8 e T9, por 5 minutos em ratos Wistar. Inicialmente, avaliou se a melhora da capacidade e da coordenação locomotora pelo teste de avaliação da capacidade locomotora (teste BBB) e da passarela gradeada nos grupos: 1- Grupo Veneno de Abelha (VB) no ponto de acupuntura - aplicação do VB na dose de 0,08 mg/kg diluído em 20μL de salina nos acupontos (E36 - Zunsanli + VG3 - Yaoyangquan); 2- Grupo VB em não ponto (VB-NP) – aplicação do VB em região que não fosse ponto de acupuntura na mesma dose que o grupo VB-E36+VG3; 3- Grupo Controle (CTL-SCI) – grupo com SCI que não foi submetido a nenhum tipo de tratamento. Nesse primeiro ensaio experimental, detectou-se que a melhora locomotora estava realmente associada à estimulação dos acupontos E36+VG3 pelo VB, já que o grupo VB-NP e o grupo CTL-SCI não apresentaram melhora significativa da capacidade locomotora parcial como o grupo VB-E36+VG3. Nossos resultados indicaram que a melhora da capacidade locomotora estava correlacionada à significativa redução da perda de tecido medular espinal promovido pela apipuntura. A próxima etapa da pesquisa investigou os tipos celulares, os fatores neuroinflamatórios, os fatores apoptóticos e a sobrevivência de neurônios e de oligodendrócitos. A apipuntura reduziu a ativação de micróglia/macrófagos no 1°, 3° e 5° dias, enquanto, a ativação de astrócitos só foi influenciada pela apipuntura no 3° dia. A apipuntura também apresentou influencia sobre a polarização de micróglia/macrófagos associados aos fenótipos pro (M1) e anti-inflamatórios (M2), no qual, a expressão de RNAm da iNOS, - marcador do fenótipo M1, foi reduzido no 3° e 5° dia e as expressões de RNAm da Arginase-1 e TGF-β, - marcadores M2, foram reduzidas apenas no 5° dia. A redução da expressão de RNAm do fator inflamatório - COX-2, influenciado pela apipuntura ocorreu no 1° e 5°dia, enquanto, a redução do NF-kB ocorreu somente no 3° dia . A apipuntura não demonstrou influencia sobre a expressão de RNAm da caspase-3 em nenhum dos tempos estudados, mas promoveu aumento significativo da expressão da proteína antiapoptótica, - o BCL-2, no 5° dia. Por fim, a apipuntura influenciou a preservação de neurônios e oligodendrócitos no tecido medular espinal no 7° dia após a SCI. Dessa forma, o conjunto de resultados sugere que a apipuntura, aplicada nos acupontos E36+VG3, foi capaz de promover recuperação parcial da locomoção e os resultados indicaram que esta melhora locomotora pode estar associada a uma influencia da apipuntura sobre a redução da ativação de astrócitos, modulação sobre a polarização de micróglia/macrófagos, sobre a redução da neuroinflamação no aumento do conteúdo da proteína antiapoptótica, o BCL-2, e na neuroproteção de neurônios e oligodendrócitos no tecido medular espinal após a SCIpor
dc.formatapplication/pdf*
dc.languageporpor
dc.publisherUniversidade Federal Rural do Rio de Janeiropor
dc.rightsAcesso Abertopor
dc.subjectApipunturapor
dc.subjectmicróglia/macrófagospor
dc.subjectpolarização M1 e M2por
dc.subjectastrócitospor
dc.subjectinflamaçãopor
dc.subjectapoptosepor
dc.subjectveneno de abelhapor
dc.subjectlesão medularpor
dc.subjectApipunctureeng
dc.subjectmicroglia / macrophageseng
dc.subjectM1 and M2 polarizationeng
dc.subjectastrocyteseng
dc.subjectinflammationeng
dc.subjectapoptosiseng
dc.subjectbee venomeng
dc.subjectspinal cord injuryeng
dc.titleA apipuntura influencia o balanço de fenótipos de micróglia/macrófagos, a neuroinflamação e o fator anti-apoptótico no modelo de lesão medular por compressão em ratos wistarpor
dc.title.alternativeThe apipuncture influences the balance of microglia / macrophages phenotypes, neuroinflammation and anti-apoptotic factor in the model of spinal cord injury by compression in wistar ratseng
dc.typeTesepor
dc.description.abstractOtherThe aim of the present study was to investigate the cell types and physiological mechanisms related to the use of apipuncture in the model of moderate spinal cord injury (SCI) by compression in Wistar rats. For induction of SCI, a fogarty embolectomy catheter was inflated with 15μL of distilled water in the extradural space, between the T8 and T9 vertebrae, for 5 minutes. Initially, it was evaluated the improvement of the locomotor capacity and coordination by locomotor rating scale (BBB test) and the grid walkway in the following groups: 1- Bee Venom Group (VB) at the acupuncture point - application of BV at the dose of 0.08 mg / kg diluted in 20μL of saline in acupoints (E36 - Zunsanli + VG3 - Yaoyangquan); 2-VB group at no point (VB-NP) - application of VB in a region that was not an acupuncture point at the same dose as the VB-E36 + VG3 group; 3 - Control Group (CTL-SCI) - group with SCI that did not undergo any type of treatment. In this first experimental trial, it was detected that the locomotor improvement was actually associated with the stimulation of the E36 + VG3 acupoints by the VB, since the VB-NP group and the CTL-SCI group did not show significant improvement of the partial locomotor capacity as the VB-E36 + VG3 group. Our results indicated that the improvement of the locomotor capacity was correlated to the significant reduction of spinal cord tissue loss promoted by apipuncture. The next step of the research investigated cell types, neuroinflammatory and apoptotic factors, and survival of neurons and oligodendrocytes. The apipuncture reduced the activation of microglia / macrophages in the 1st, 3rd and 5th days, whereas, the activation of astrocytes was only influenced by apipuncture on the 3rd day. The apipuncture also had an influence on microglia / macrophage polarization associated with pro (M1) and anti-inflammatory (M2) phenotypes, in which iNOS mRNA expression - marker of the M1 phenotype was reduced in the 3rd and 5th Day and Arginase-1 and TGF-β (M2 markers) mRNA expression were reduced only on the 5th day. The reduction of COX-2 (inflammatory factor) mRNA expression influenced by apipuncture occurred on day 1 and 5, while the reduction of NF-kB occurred only on day 3. The apipuncture showed no influence over caspase-3 mRNA expression in any of the analysed times, but promoted a significant increase in the expression of the anti-apoptotic protein BCL-2 on the 5th day. Finally, apipuncture influenced the preservation of neurons and oligodendrocytes in spinal cord tissue on the 7th day after SCI. Thus, the set of results suggests that apipuncture, applied in E36 + VG3 acupoints, was able to promote partial recovery of locomotion. This locomotor improvement may be associated with an influence of apipuncture on the reduction of astrocyte activation, modulation on microglial / macrophage polarization, reduction of neuroinflammation through the increase of anti-apoptotic protein content, BCL-2, and neuroprotection of neurons and oligodendrocytes in spinal cord tissue after SCI.eng
dc.contributor.advisor1Medeiros, Magda Alves de
dc.contributor.advisor1ID096.592.487-38por
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/6392136073564306por
dc.contributor.referee1Medeiros, Magda Alves de
dc.contributor.referee2Allodi, Silvana
dc.contributor.referee3Resende, Victor Túlio Ribeiro de
dc.contributor.referee4Malvar, David do Carmo
dc.contributor.referee5Santiago, Marcelo Felippe
dc.creator.ID110.603.277-24por
dc.creator.Latteshttp://lattes.cnpq.br/3151736859168960por
dc.publisher.countryBrasilpor
dc.publisher.departmentInstituto de Ciências Biológicas e da Saúdepor
dc.publisher.initialsUFRRJpor
dc.publisher.programPrograma Multicêntrico de Pós-Graduação em Ciências Fisiológicaspor
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dc.subject.cnpqFisiologiapor
dc.thumbnail.urlhttps://tede.ufrrj.br/retrieve/65204/2017%20-%20Raquel%20do%20Nascimento%20de%20Souza.pdf.jpg*
dc.originais.urihttps://tede.ufrrj.br/jspui/handle/jspui/4675
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