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Please use this identifier to cite or link to this item: https://rima.ufrrj.br/jspui/handle/20.500.14407/10638
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dc.contributor.authorMichel, Daniele Cabral
dc.date.accessioned2023-12-22T01:40:27Z-
dc.date.available2023-12-22T01:40:27Z-
dc.date.issued2017-02-14
dc.identifier.citationMICHEL, Daniele Cabral. Caracterização de estirpes de Bradyrhizobium isoladas de Centrolobium paraense. 2017. 67 f. Dissertação (Mestrado em Agronomia - Ciência do Solo). Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2017.por
dc.identifier.urihttps://rima.ufrrj.br/jspui/handle/20.500.14407/10638-
dc.description.abstractCentrolobium paraense Tul. popularmente conhecido como “pau-rainha”, é uma espécie de leguminosa que ocorre naturalmente no norte do estado de Roraima (Brasil) ao Panamá. Tem importância econômica, social e ambiental para populações locais, sendo uma espécie fixadora de nitrogênio (N) em associação com bactérias do grupo dos rizóbios. Treze bactérias gram-negativas, aeróbicas, com mobilidade através de flagelos polares e em forma de bastonetes previamente isoladas de nódulos de raízes de Centrolobium paraense cultivadas em solos do estado de Roraima foram submetidas a caracterização com abordagem polifásica. O estudo envolveu a utilização de fontes de carbono, reações enzimáticas, composição de ácidos graxos das células, antibióticos, pH e tolerância a NaCl,16S rRNA, análise filogenética dos genes nodC, nifH e de sequências multilocus (MLSA com dnaK, glnII, recA, gyrB e rpoB), conteúdo de G+C e identidade média de nucleotídeos (ANI). O crescimento das estirpes foi observado num intervalo de temperatura 20-36°C (ótima 28°C), intervalo de pH de 5-11 (ótimo 6,0-7,0) e 0,1-0,5% NaCl (ótimo 0,1-0,3%). A análise do gene 16S rRNA posicionou as estirpes em dois grupos dentro de Bradyrhizobium. A espécie mais próxima (98,8%) para o grupo I foi B. neotropicale enquanto para o grupo II foram outras doze espécies com mais de 99% de similaridade. MLSA confirmou B. neotropicale BR 10247T como a estirpe tipo mais próxima do grupo I e B. elkanii USDA 76T e B. pachyrhizi PAC 48T do grupo II. O ANI diferenciou o grupo I da B. neotropicale BR 10247T (79,6%) e o grupo II da B. elkanii USDA 76T e B. pachyrhizi PAC 48T (88,1 e 87,9 respectivamente. O perfil de ácidos graxos (predominância de C16:0 e caracteristíca somada 8 (18:1ω6c/18:1ω7c) para ambos os grupos, ANI, o conteúdo de G+C e a utilização de compostos de carbono deram suporte ao posicionamento das novas estirpes no gênero Bradyrhizobium. Os genes nodC e nifH têm em geral baixa similaridade com outras espécies de Bradyrhizobium. Ambos os grupos nodularam plantas de diferentes tribos.por
dc.description.sponsorshipCAPESpor
dc.description.sponsorshipFAPERJpor
dc.formatapplication/pdf*
dc.languageporpor
dc.publisherUniversidade Federal Rural do Rio de Janeiropor
dc.rightsAcesso Abertopor
dc.subjectBNFeng
dc.subjectTaxonomyeng
dc.subjectPhylogenyeng
dc.subjectFBNpor
dc.subjectTaxonomiapor
dc.subjectFilogeniapor
dc.titleCaracterização de estirpes de Bradyrhizobium isoladas de Centrolobium paraensepor
dc.title.alternativeCaracterization of strains isolated from Centrolobium paraenseeng
dc.typeDissertaçãopor
dc.description.abstractOtherCentrolobium paraense Tul. popularly known as "pau-rainha", is a naturally occurring legume species from the northern state of Roraima (Brazil) to Panama. It has economic, social and environmental importance for local populations, being a nitrogen fixing species in association with bacteria of the rhizobia group. Thirteen Gram-negative, aerobic, motile with polar flagella, rods shaped bacteria previously isolated from root nodules of C. paraense grown in soils from the Roraima State were submitted to a polyphasic approach characterization. The study involved the carbon source utilization, enzymatic reaction, antibody, pH and NaCl tolerance, 16S rRNA, nodC, nifH and multi locus sequence phylogenetic analysis (MLSA with dnaK, glnII, recA, gyrB and rpoB), G+C content and Average Nucleotide Identity (ANI). Growth of strains was observed at temperature range 20- 36°C (optimal 28°C), pH ranges 5-11 (optimal 6.0-7.0) and 0.1-0.5%NaCl (optimal 0.1- 0.3%). Analysis of 16S rRNA gene placed the strains into two groups within Bradyrhizobium. Closest neighbouring species (98.8%) for group I was B. neotropicale while for group II were twelve species with more than 99% of similarity. MLSA confirmed B. neotropicale BR 10247T as the closest type strain for the group I and B. elkanii USDA 76T and B. pachyrhizi PAC 48T for group II. ANI differentiated group I from the B. neotropicale BR 10247T (79.6%), and group II from B. elkanii USDA 76T and B. pachyrhizi PAC 48T (88.1% and 87.9%, respectively). Fatty acid profiles (majority C16:0 and Summed feature 8 (18:1ω6c/18:1ω7c) for both groups), DNA G+C content and carbon compound utilization supported the placement of the novel strains in the genus Bradyrhizobium. Gene nodC and nifH of the new strains have in general low similarity with other Bradyrhizobium species. Both groups nodulated plants from the tribes Crotalarieae, Dalbergiae, Genisteae and Phaseoleae. Based on the result obtained, two novel species which the names Bradyrhizobium centrolobii and Bradyrhizobium macuxiense are proposed.eng
dc.contributor.advisor1Zilli, Jerri Édson
dc.contributor.advisor1ID927.881.579-91por
dc.contributor.referee1Zilli, Jerri Édson
dc.contributor.referee2Schultz, Nivaldo
dc.contributor.referee3Botelho, Glória Regina
dc.contributor.referee4Coelho, Irene da Silva
dc.creator.ID112.148.507-31por
dc.creator.Latteshttp://lattes.cnpq.br/5016121544662212por
dc.publisher.countryBrasilpor
dc.publisher.departmentInstituto de Agronomiapor
dc.publisher.initialsUFRRJpor
dc.publisher.programPrograma de Pós-Graduação em Agronomia - Ciência do Solopor
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