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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Lima, Nathanielly Rocha Casado de | |
| dc.date.accessioned | 2023-12-22T01:42:54Z | - |
| dc.date.available | 2023-12-22T01:42:54Z | - |
| dc.date.issued | 2011-03-30 | |
| dc.identifier.citation | LIMA, Nathanielly Rocha Casado de. Análise do crescimento, diferenciação celular e perfil isoenzimático de um isolado de tripanosomatídeo obtido de Megaselia scalaris Loew, 1866 (Díptera, Phoridae). 2011. 54 f. Dissertação (Mestrado em Biologia Animal) - Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2011. | por |
| dc.identifier.uri | https://rima.ufrrj.br/jspui/handle/20.500.14407/10768 | - |
| dc.description.abstract | A família Trypanosomatidae inclui parasitas de uma grande variedade de vertebrados, invertebrados (principalmente insetos), plantas e algumas espécies de protozoários. No presente trabalho, um isolado foi obtido por coprocultivo de trato intestinal de Megaselia scalaris Loew, 1866 (Diptera, Phoridae). O isolado original e seus clones (obtidos por citometria de fluxo) foram depositados na "Coleção de Tripanosomatídeos do Instituto Oswaldo Cruz". Estes clones foram caracterizados por diversas abordagens em comparação com espécies de referência de diferentes gêneros. Seu crescimento foi estudado em meio LIT (com 10 ou 20% de soro fetal bovino) modificado, de acordo com o nível de exigência. O número de células por microlitro foi estimado em intervalos de 24 h, entre 48-144 h. Esfregaços corados pelo Giemsa (após hidrólise ácida) foram preparados visando estudos de diferenciação celular e morfometria das formas encontradas. A análise de isoenzimas foi realizada utilizando-se os seguintes sistemas: GPI , PGM, 6PGDH, ME, IDH, MDH, ACON, FUM, MPI e HK. Os dados desta análise foram processados numericamente e submetidos à análise computacional utilizando-se o coeficiente de Jaccard e o algoritmo UPGMA. O conjunto de resultados deste trabalho sugere que: (1) O isolado de M. scalaris apresentou similaridade de 70% com Herptomonas megaseliae; (2) Esse isolado revelou a presença exclusiva de promastigotas em toda análise da curva de crescimento, como esperado para o gênero Leptomonas, entretanto as formas exibiram grande polimorfismo. Estudos complementares utilizando ferramentas moleculares serão realizados. | por |
| dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES | por |
| dc.format | application/pdf | * |
| dc.language | por | por |
| dc.publisher | Universidade Federal Rural do Rio de Janeiro | por |
| dc.rights | Acesso Aberto | por |
| dc.subject | Tripanosomatídeos inferiores | por |
| dc.subject | morfobiologia e isoenzimas | por |
| dc.title | Análise do crescimento, diferenciação celular e perfil isoenzimático de um isolado de tripanosomatídeo obtido de Megaselia scalaris Loew, 1866 (Díptera, Phoridae) | por |
| dc.title.alternative | Analysis of growth, cell differentiation and isoenzyme profile of an isolated trypanosomatid obtained Megaselia scalaris Loew, 1866 (Diptera, Phoridae) | eng |
| dc.type | Dissertação | por |
| dc.description.abstractOther | The Trypanosomatidae family includes parasites of a wide variety of vertebrates, invertebrates (mainly insects), plants and some species of protozoa. In this study, an isolate was obtained by feces culturing of the intestinal tract of Megaselia scalaris Loew, 1866 (Diptera, Phoridae). The original isolate and its clones (obtained by flow cytometry) were deposited in the ―Trypanosomatids Collection of the Instituto Oswaldo Cruz. These clones were characterized by different approaches I in comparison with the reference species of different genera. Its growth has been studied in LIT medium (with 10 or 20% fetal bovine serum) modified according to the requirement. The number of cells per microliter was estimated at 24 h intervals between 48-144 h. Giemsa-stained smears (after acid hydrolysis) were prepared in order to study cell differentiation and morphology of the forms. The isozyme analysis was performed using the following systems: GPI, PGM, 6PGDH, ME, IDH, MDH, ACON, FUM, MPI and HK. Data from this analysis were processed and analyzed numerically using the computacional Jaccard coefficient and UPGMA algorighm. The result set of this study suggest that: (1) The strain of M. scalaris showed 70% similarity with Herpetomonas megaseliae, (2) This isolate showed the exclusive presence of promastigotes in any analysis of the growth curve as expected for the genus Leptomonas, however the forms exhibited high polymorphism. Additional studies using molecular tools will be realized | eng |
| dc.contributor.advisor1 | Mallet, Jacenir Reis dos Santos | |
| dc.contributor.advisor1ID | 710.008.957-34 | por |
| dc.contributor.advisor1Lattes | http://lattes.cnpq.br/9643185827631520 | por |
| dc.contributor.referee1 | Gomes, Suzete Araújo Oliveira | |
| dc.contributor.referee2 | Gonçalves, Teresa Cristina Monte | |
| dc.creator.ID | 8207359730 | por |
| dc.creator.Lattes | http://lattes.cnpq.br/3757620013031888 | por |
| dc.publisher.country | Brasil | por |
| dc.publisher.department | Instituto de Ciências Biológicas e da Saúde | por |
| dc.publisher.initials | UFRRJ | por |
| dc.publisher.program | Programa de Pós-Graduação em Biologia Animal | por |
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Discovery and barcoding by analysis of Spliced Leader RNA gene sequences of new isolates of trypanosomatidae from heteroptera in Costa Rica. J Euk Microbiol 54(1): 57-65 McGhee RB & Hanson WL 1963. The relationship of various crithidias as determined by serological reactions. J Protozool 18: 80-81. McGhee RB & Cosgrove WB 1980. Biology and physiology of the lower Trypanosomatidae. Microbiol Rev 44: 140-173. 48 Muller E, Gargani D, Schaeffer V, Stevens J, Fernandez-Becerra C, Sanchez-Moreno M, Dollet M 1994. Variability in the phloem resctricted plant trypanosomes (Phytomonas spp.) associated with wilts of cultivated crops. Isoenzyme comparison with the lower trypanosomatids. Eur J P Pathol 100: 425-434. Pacheco RS, Marzochi MCA, Pires MQ, Brito CMM, Madeira MF, Barbosa-Santos EGO 1998. Parasite genotypically related to a monoxenous trypanosomatid of dog’s flea causing opportunistic infection in an HIV positive patient. Mem Inst Oswaldo Cruz 93: 531-537. Patton WS 1908. 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Developmental stages of trypanosomatid flagellates: a new terminology revisited. Protozoology 3: 51-53. 51 Wallace FG 1979. Biology of the Kinetoplastida of arthropods, p 213-240. In: WHR Lumsden, DA Evans (eds), Biology of the kinetoplastida, vol 2, Academic Press, London. Wenyon CM 1926. Protozoology. A manual for medical men, veterinarians and zoologists, vol 1, p. 155-468, Baillière, Tindall & Cox, London. | por |
| dc.subject.cnpq | Parasitologia | por |
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| dc.originais.uri | https://tede.ufrrj.br/jspui/handle/jspui/1224 | |
| dc.originais.provenance | Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-09-28T15:23:40Z No. of bitstreams: 1 2011 - Nathanielly Rocha Casado de Lima.pdf: 972650 bytes, checksum: 29dba077fa4bb5ed1cb4977f2f174d44 (MD5) | eng |
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| Appears in Collections: | Mestrado em Biologia Animal | |
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