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dc.contributor.authorTolomelli, Roberta Caroline Alvarenga
dc.date.accessioned2023-12-22T01:57:18Z-
dc.date.available2023-12-22T01:57:18Z-
dc.date.issued2017-08-16
dc.identifier.citationTolomelli, Roberta Caroline Alvarenga. Detecção molecular de Babesia vogeli (Piroplasmida: Babesiidae) e Ehrlichia canis (Rickettsiales: Anaplasmataceae) em carrapatos não ingurgitados coletados em ambiente peri-domiciliar em dois municípios da microrregião de Itaguaí, Rio de Janeiro. 2017. [50 f.]. Dissertação( Programa de Pós-Graduação em Ciências Veterinárias) - Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, [Seropédica-RJ] .por
dc.identifier.urihttps://rima.ufrrj.br/jspui/handle/20.500.14407/11813-
dc.description.abstractAs babesioses e erliquioses caninas são transmitidas pelo carrapato Rhipicephalus sanguineus sensu lato (s.l.). Estes hemoparasitos são bem distribuídos no país, sendo a erliquiose mais frequente que babesiose. O objetivo deste trabalho foi avaliar a frequência de Ehrlichia canis e Babesia vogeli em carrapatos não alimentados coletados em dois municípios da microrregião de Itaguaí, Rio de Janeiro. Foram analisados 369 carrapatos R. sanguineus s.l., incluindo adultos (194), ninfas (92) e larvas (83). As amostras foram submetidos à extração de DNA pelo método de HotSHOT modificado. A detecção molecular de E. canis foi realizada através da reação em cadeia da polimerase (PCR) com alvo no gene “Disulfide oxidoreductase” (dsb) de Ehrlichia spp., amplificando um fragmento de 409 pares de bases (pb). Para detecção de Babesia vogeli, as amostras foram testadas em uma PCR que amplifica um fragmento de 523pb do gene “heat shock protein 70kDa” (hsp70) de B. vogeli. Foi obtido um total de 41 (11,11%) amostras positivas para E. canis, sendo 11 (5,67%) adultos, 8 (8,69%) ninfas e 22 (26,5%) larvas (p<0,05). Em relação a B. vogeli foram observadas 24 (6,5%) de amostras positivas, sendo 9 (4,63%) adultos, 7 (7,6%) ninfas e 8 (9,63%) larvas. As análises epidemiológicas demonstraram que a frequência de B. vogeli foi maior na zona rural do que urbana (p<0,05), e também em locais onde os cães possuíam abrigos (P<0,05). Concluiu-se que os locais de maior prevalência de B. vogeli na região estão contidos mais na zona rural do que urbana e também onde há presença de abrigo para os cães. Na detecção de E. canis foi observada maior frequência do hemoparasito em Itaguaí do que Seropédica (p=0,01). O presente estudo demonstrou E. canis em larvas de R. sanguineus s.l. não alimentadas, reforçando a possibilidade de transmissão transovariana da bactéria, porém, são necessários mais estudos para demonstrar essa forma de transmissão no vetorpor
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESpor
dc.formatapplication/pdf*
dc.languageporpor
dc.publisherUniversidade Federal Rural do Rio de Janeiropor
dc.rightsAcesso Abertopor
dc.subjectHemoparasitospor
dc.subjectRhipicephalus sanguineus sensu latopor
dc.subjectcarrapatopor
dc.subjectepidemiologiapor
dc.subjectinfecção naturalpor
dc.subjectHemoparasiteseng
dc.subjectRhipicephalus sanguineus sensu latoeng
dc.subjecttickeng
dc.subjectepidemiologyeng
dc.subjectnatural infectioneng
dc.titleDetecção molecular de Babesia vogeli (Piroplasmida: Babesiidae) e Ehrlichia canis (Rickettsiales: Anaplasmataceae) em carrapatos não ingurgitados coletados em ambiente peri-domiciliar em dois municípios da microrregião de Itaguaí, Rio de Janeiropor
dc.title.alternativeMolecular detection of Babesia vogeli (Piroplasmida: Babesiidae) and Ehrlichia canis (Rickettsiales: Anaplasmataceae) in unfed ticks collected in peridomiciliary environment in two countries of Itaguaí microregion, Rio de Janeiroeng
dc.typeDissertaçãopor
dc.description.abstractOtherCanine babesiosis and ehrlichiosis are transmitted by the brown dog tick, Rhipicephalus sanguineus sensu lato (s.l.). These hemoparasites are well distributed in Brazil and the canine ehrlichiosis is more frequent than babesiosis. The aim of this study was to assess the frequency of Ehrlichia canis and Babesia vogeli in unfed ticks from two counties of Itaguaí microregion, Rio de Janeiro. 369 R. sanguineus s.l. ticks were analyzed in peridomiciliary environment, including adults (194), nymphs (92) and larvae (83). The DNA samples were extracted through a HotSHOT modified method. The molecular detection of E. canis was made by a PCR that amplifies a 409 bp fragment of Disulfide oxidoreductase (dsb) gene of Ehrlichia spp. For B. vogeli detection, the samples were tested by a PCR that amplifies a 523 bp fragment of heat shock protein 70kDa (hsp70) gene. We obtained 41 (11.11%) positive samples for E. canis, 11 (5,67%) adults, 8 (8,69%) nymphs ans 22 (26,5%) larvae (p<0.05). From PCR of B vogeli, we obtained 24 (6,5%) positive samples, 9 (4,63%) adults, 7 (7,6%) nymphs and 8 (9,63%) larvae. The bivariate analysis showed significance for the presence of dog’s shelter in relation to B. vogeli infection in ticks (p<0.05). The area of collection had statistical significance too. It demonstrated that ticks in rural areas are more likely to get infection by B. vogeli (p<0.05). We concluded that B. vogeli is more prevalent in rural areas and areas which the dog lives with shelter. The analysis of E. canis samples showed that the hemoparasite is more frequent in Itaguaí city than Seropédica (p=0,01). This study has shown the presence of E. canis in larvae of R. sanguineus s.l.. This event increases the possibility of transovarial transmission of the bacteria, however, more studies are necessary to demonstrate experimentally this kind of transmission in the tick vectoreng
dc.contributor.advisor1Santos, Huarrisson Azevedo
dc.contributor.advisor1ID98383329504por
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/3751609492049306por
dc.contributor.advisor-co1Peixoto, Maristela Peckle
dc.contributor.advisor-co1ID11079501711por
dc.contributor.advisor-co1Latteshttp://lattes.cnpq.br/8817867478588076por
dc.contributor.referee1Santos, Huarrisson Azevedo
dc.contributor.referee2Silva, Claudia Bezerra da
dc.contributor.referee3Cunha, Nathalie Costa da
dc.creator.ID34653578842por
dc.creator.Latteshttp://lattes.cnpq.br/0017401529431755por
dc.publisher.countryBrasilpor
dc.publisher.departmentInstituto de Veterináriapor
dc.publisher.initialsUFRRJpor
dc.publisher.programPrograma de Pós-Graduação em Ciências Veterináriaspor
dc.relation.referencesADACHI, K.; TATEISHI, M.; HORII, Y.; NAGATOMO, H.; SHIMIZU, T.; MAKIMURA, S. Immunologic characteristics of anti-erythrocyte membrane antibody produced in dogs during Babesia gibsoni infection. Journal of Veterinary Med Sci, v. 57, n. 1, p. 121–123, 1995. AGUIAR, D. M.; CAVALCANTE, G. T.; PINTER, A.; GENNARI, S. M.; CAMARGO, L. M. A.; LABRUNA, M. B. Prevalence of Ehrlichia canis (Rickettsiales : Anaplasmataceae) in dogs and Rhipicephalus sanguineus (Acari : Ixodidae) ticks from Brazil. Journal of Medical Entomology, v. 44, n. 1, p. 126–132, 2007. ALASAAD, S.; ROSSI, L.; MAIONE, S.; SARTORE, S.; SORIGUER, R. C.; PÉREZ, J. M.; RASERO, R.; ZHU, X. Q.; SOGLIA, D. HotSHOT Plus ThermalSHOCK , a new and efficient technique for preparation of PCR-quality mite genomic DNA. Parasitology Research, v. 103, p. 1455–1457, 2008. ALCAIDE, M.; RICO, C.; RUIZ, S.; SORIGUER, R.; MUÑOZ, J.; FIGUEROLA, J. Disentangling vector-borne transmission networks : A universal DNA barcoding method to identify vertebrate hosts from arthropod bloodmeals. PloS one, v. 4, n. 9, p. 1–6, 2009. ALMOSNY, N. R. P. Ehrlichia canis (DONATIEN & LESTOQUARD, 1935): Avaliação Parasitológica, Hematológica e Bioquímica Sérica da Fase Aguda de Cães e Gatos Experimentalmente Infectados. 1998. Universidade Federal Rural do Rio de Janeiro. AMMAZZALORSO, A. D.; ZOLNIK, C. P.; DANIELS, T. J.; KOLOKOTRONIS, S.-O. To beat or not to beat a tick : comparison of DNA extraction methods for ticks (Ixodes scapularis). PeerJ, p. 1–14, 2015. ANDRÉ, M. R. Detecção molecular e sorológica de Ehrlichia canis e Babesia canis em felídeos selvagens brasileiros mantidos em cativeiro. 2008. Universidade Estadual Paulista, 2008. ARAUJO, A. C.; SILVEIRA, J. A. G.; AZEVEDO, S. S.; NIERI-BASTOS, F. A.; RIBEIRO, M. F. B.; LABRUNA, M. B.; HORTA, M. C. Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernambuco, Brazil. Pesquisa Veterinaria Brasileira, v. 35, n. 5, p. 456–461, 2015. ARMAS, Y. de; RODRÍGUEZ, M. M.; BISSET, J. A. Modification of a method to extract genomic DNA from Aedes aegypti (Diptera: Culicidae). Revista Colombiana, v. 31, p. 203– 206, 2005. AYOOB, A. L.; HACKNER, S. G.; PRITTIE, J. Clinical management of canine babesiosis. Journal of Veterinary Emergency and Critical Care, v. 20, n. 1, p. 77–89, 2010. BANETH, G. Tick-borne infections of animals and humans : A common ground. International Journal for Parasitology, v. 44, n. 9, p. 591–596, 2015. 38 BARKER, E. N.; LANGTON, D. A.; HELPS, C. R.; BROWN, G.; MALIK, R.; SHAW, S. E.; TASKER, S. Haemoparasites of free-roaming dogs associated with several remote Aboriginal communities in Australia. Veterinary Research, v. 55, n. 8, p. 1–7, 2012. BARREIRA, J. D. Efeitos da infecção de Babesia bigemina (smith & kilborne, 1893) e Babesia bovis (Babés, 1888) sobre os parâmetros biológicos do carrapato Boophilus microplus (Canestrini, 1887). 2001. Universidade Federal Rural do Rio de Janeiro, 2001. BARROS-BATTESTI, D. M. Carrapatos de Importância Médico-Veterinária da Região Neotropical: Um guia ilustrado para identificação de espécies. São Paulo: ICTTD, 2006. BICALHO, K. A.; RIBEIRO, M. F. B.; MARTINS-FILHO, O. A. Molecular fluorescent approach to assessing intraerythrocytic hemoprotozoan Babesia canis infection in dogs. Veterinary Parasitology, v. 125, n. 3–4, p. 221–235, 2004. BÖHM, M.; LEISEWITZ, A. L.; THOMPSON, P. N.; SCHOEMAN, J. P. Capillary and venous Babesia canis rossi parasitaemias and their association with outcome of infection and circulatory compromise. Veterinary Parasitology, v. 141, n. 1–2, p. 18–29, 2006. BOOZER, A. L.; MACINTIRE, D. K. Canine babesiosis. Veterinary Clinics of North America: Small Animal Practice, v. 33, n. 4, p. 885–904, jul. 2003. BOTELHO, M. de C.; MACHADO, R. Z.; AQUINO, L. P. C. T. de; ALESSI, A. C.; COSTA, M. T. Experimental acute canine monocytic ehrlichiosis : clinicopathological and immunopathological findings. Veterinary Parasitology, v. 119, p. 73–86, 2004. BREMER, W. G.; SCHAEFER, J. J.; WAGNER, E. R.; MOORE, D. L.; STICH, R. W. Transstadial and intrastadial experimental transmission of Ehrlichia canis by male Rhipicephalus sanguineus. Veterinary Parasitology, v. 131, p. 95–105, 2005. CACCIÒ, S.; CAMMÀ, C.; ONUMA, M.; SEVERINI, C. The B-tubulin gene of Babesia and Theileria parasites is an informative marker for species discrimination. International Journal for Parasitology, v. 30, p. 0–4, 2000. CARCY, B.; RANDAZZO, S.; DEPOIX, D.; ADASZEK, L.; CARDOSO, L.; BANETH, G.; GORENFLOT, A.; SCHETTERS, T. P. Classification of Babesia canis strains in Europe based on polymorphism of the Bc28 1 -gene from the Babesia canis Bc28 multigene family. Veterinary Parasitology, v. 211, n. 3–4, p. 111–123, 2015. CARDOSO, L.; TUNA, J.; VIEIRA, L.; YISASCHAR-MEKUZAS, Y.; BANETH, G. Molecular detection of Anaplasma platys and Ehrlichia canis in dogs from the North of Portugal. The Veterinary Journal, v. 183, n. 2, p. 232–233, 2010. CARNOHAN, L. P.; KAUFMAN, P. E.; ALLAN, S. A.; GEZAN, S. A.; WEEKS, E. N. I. Evaluation of four bed bug traps for surveillance of the brown dog tick ( Acari : Ixodidae ). Journal of Medical Entomology, p. 1–9, 2015. CARRET, C.; WALAS, F.; CARCY, B.; GRANDE, N.; PRÉCIGOUT, É.; MOUBRI, K.; SCHETTERS, T. P.; GORENFLOT, A. Babesia canis canis, Babesia canis vogeli, Babesia canis rossi: Differentiation of the three subspecies by a restriction fragment length polymorphism analysis on amplified small subunit ribosomal RNA genes. v. 46, n. 3, p. 298– 39 303, 1999. CARVALHO, F. S.; WENCESLAU, A. A.; CARLOS, R. S. A.; ALBUQUERQUE, G. R. Epidemiological and molecular study of Ehrlichia canis in dogs in Bahia , Brazil. Genetics and Molecular Research, v. 7, n. 3, p. 657–662, 2008. CONRATHS, F. J.; SCHARES, G. Validation of molecular-diagnostic techniques in the parasitological laboratory. Veterinary Parasitology, v. 136, p. 91–98, 2006. CORALES, J. M. I.; VILORIA, V. V; VENTURINA, V. M.; MINGALA, C. N. The prevalence of Ehrlichia canis , Anaplasma platys and Babesia spp . in dogs in Nueva Ecija , Philippines based on multiplex polymerase chain reaction ( mPCR ) assay. Annals of Parasitology, v. 60, n. 4, p. 267–272, 2014. COSTA-JÚNIOR, L. M.; REMBECK, K.; PASSOS, L. M. F.; RIBEIRO, M. F. B. Factors associated with epidemiology of Anaplasma platys in dogs in rural and urban areas of Minas Gerais State , Brazil. Preventive Veterinary Medicine, v. 109, p. 321–326, 2013. COSTA-JÚNIOR, L. M.; ZAHLER-RINDER, M.; RIBEIRO, M. F. B.; REMBECK, K.; RABELO, E. M. L.; PASSOS, L. M. F. Use of a Real Time PCR for detecting subspecies of Babesia canis. Veterinary Parasitology, v. 188, n. 1–2, p. 160–163, 2012. COSTA, A. P. da; COSTA, F. B.; LABRUNA, M. B.; SILVEIRA, I.; MORAES-FILHO, J.; SOARES, J. F.; SPOLIDORIO, M. G.; GUERRA, R. de M. S. N. de C. G. A serological and molecular survey of Babesia vogeli , Ehrlichia canis and Rickettsia spp. among dogs in the state of Maranhão , northeastern Brazil. Brazilian Journal of Veterinary Parasitology, v. 24, n. 1, p. 28–35, 2015. CROWDER, C. D.; ROUNDS, M. A.; PHILLIPSON, C. A.; PICURI, J. M.; MATTHEWS, H. E.; HALVERSON, J.; SCHUTZER, S. E.; ECKER, D. J.; ESHOO, M. W.; CROWDER, C. D.; ROUNDS, M. A.; PHILLIPSON, C. A.; PICURI, J. M.; MATTHEWS, H. E.; HALVERSON, J.; SCHUTZER, S. E.; ECKER, D. J. Extraction of total nucleic acids from ticks for the detection of bacterial and viral pathogens. Vector-Borne and Zoonotic Diseases, v. 47, n. 1, 2010. DAGNONE, A. S.; DE MORAIS, H. S. A.; VIDOTTO, M. C.; JOJIMA, F. S.; VIDOTTO, O. Ehrlichiosis in anemic, thrombocytopenic, or tick-infested dogs from a hospital population in South Brazil. Veterinary Parasitology, v. 117, n. 4, p. 285–290, 2003. DAGNONE, A. S.; MORAIS, H. S. A. de; VIDOTTO, O. Erliquiose nos animais e no homem. Semina: Ciências Agrárias, v. 22, n. 2, p. 191–201, 2001. DANTAS-TORRES, F. Canine vector-borne diseases in Brazil. Parasites & Vectors, v. 25, n. 1, p. 1–17, 2008. DANTAS-TORRES, F. Biology and ecology of the brown dog tick , Rhipicephalus sanguineus. Parasites & Vectors, v. 3, n. 26, p. 1–11, 2010. DANTAS-TORRES, F.; FIGUEREDO, L. A.; OTRANTO, D. Seasonal variation in the effect of climate on the biology of Rhipicephalus sanguineus in southern Europe. Parasitology, v. 138, n. 4, p. 527–536, 2011. 40 DANTAS-TORRES, F.; LATROFA, M. S.; ANNOSCIA, G.; GIANNELLI, A.; PARISI, A.; OTRANTO, D. Morphological and genetic diversity of Rhipicephalus sanguineus sensu lato from the New and Old Worlds. Parasites & Vectors, v. 213, 2013. DHARMARAJAN, G.; RHODES, O. E. Evaluating levels of PCR efficiency and genotyping error in DNA extracted from engorged and non-engorged female Dermacentor variabilis ticks. Medical and Veterinary Entomology, v. 25, p. 109–112, 2011. DOYLE, C. K.; LABRUNA, M. B.; BREITSCHWERDT, E. B.; TANG, Y.-W.; CORSTVET, R. E.; HEGARTY, B. C.; BLOCH, K. C.; LI, P.; WALKER, D. H.; MCBRIDE, J. W. Detection of medically important Ehrlichia by quantitative multicolor TaqMan Real- Time Polymerase Chain Reaction of the dsb gene. The Journal of Molecular Diagnostics, v. 7, n. 4, p. 504–510, 2005. DUARTE, S. C.; LINHARES, G. F. C.; ROMANOWSKY, T. N.; NETO, O. J. da S.; BORGES, L. M. F. Assessment of primers designed for the subspecies-specific discrimination among Babesia canis canis , Babesia canis vogeli and Babesia canis rossi by PCR assay. Veterinary Parasitology, v. 152, p. 16–20, 2008. FERROLHO, J.; SIMPSON, J.; HAWES, P.; ZWEYGARTH, E.; BELL-SAKYI, L. Growth of Ehrlichia canis, the causative agent of canine monocytic ehrlichiosis, in vector and nonvector ixodid tick cell lines. Ticks and Tick-borne Diseases, v. 7, n. 4, p. 631–637, 2016. FOURIE, J. J.; STANNECK, D.; LUUS, H. G.; BEUGNET, F.; WIJNVELD, M.; JONGEJAN, F. Transmission of Ehrlichia canis by Rhipicephalus sanguineus ticks feeding on dogs and on artificial membranes. Veterinary Parasitology, v. 197, n. 3–4, p. 595–603, 2013. FOURNIER, P.-E.; DURAND, J.-P.; ROLAIN, J.-M.; CAMICAS, J.-L.; TOLOU, H.; RAOULT, D. Detection of Astrakhan fever rickettsia from ticks in Kosovo. Annals of the New York Academy of Sciences, v. 990, p. 158–61, 2003. FRYXELL, R. T. T.; STEELMAN, C. D.; SZALANSKI, A. L.; BILLINGSLEY, P. M.; WILLIAMSON, P. C. Molecular detection of Rickettsia species within ticks (acari: Ixodidae) collected from Arkansas United States. Journal of Medical Entomology, v. 52, n. 3, p. 500– 508, 2015. FUKUMOTO, S.; XUAN, X.; INOUE, N.; IGARASHI, I.; SUGIMOTO, C.; FUJISAKI, K.; NAGASAWA, H.; MIKAMI, T.; SUZUKI, H. Molecular characterization of a gene encoding a 29-kDa cytoplasmic protein of Babesia gibsoni and evaluation of its diagnostic potentiality. Molecular & Biochemical Parasitology, v. 131, p. 129–136, 2003. GAUNT, S. D.; BEALL, M. J.; STILLMAN, B. A.; LORENTZEN, L.; DINIZ, P.; CHANDRASHEKAR, R.; BREITSCHWERDT, E. B. Experimental infection and coinfection of dogs with Anaplasma platys and Ehrlichia canis : hematologic , serologic and molecular findings. Parasites & Vectors, v. 33, n. 3, p. 1–10, 2010. GHAFAR, M. W.; AMER, S. A. Prevalence and first molecular characterization of Anaplasma phagocytophilum, the agent of human granulocytic anaplasmosis, in Rhipicephalus sanguineus ticks attached to dogs from Egypt. Journal of Advanced 41 Research, v. 3, n. 2, p. 189–194, 2012. GHIRBI, Y. M.; GHORBEL, A.; AMOURI, M.; NEBAOUI, A.; HADDAD, S.; BOUATTOUR, A. Clinical , serological , and molecular evidence of ehrlichiosis and anaplasmosis in dogs in Tunisia. Parasitology Research, v. 104, p. 767–774, 2009. GONÇALVES, L. R.; FILGUEIRA, K. D.; AHID, S. M. M.; PEREIRA, J. S.; DO VALE, A. M.; MACHADO, R. Z.; ANDRÉ, M. R. Study on coinfecting vector-borne pathogens in dogs and ticks in Rio Grande do Norte , Brazil. Brazilian Journal of Veterinary Parasitology, v. 23, n. 3, p. 407–412, 2014. GREENE, C. E. INFECTIOUS DIESEASES OF THE DOG AND CAT. [s.l.] ELSEVIER, 2012. GROVEZ, M. G.; DENNIS, G. L.; AMYX, H. L.; HUXSOLL, D. L. Transmission of Ehrlichia canis to dogs by ticks (Rhipicephalus sanguineus). American Journal of Veterinary Research, v. 36, n. 7, p. 937–940, 1975. GUY, E. C.; STANEK, G. Detection of Borrelia burgdorferi in patients with Lyme disease by the polymerase chain reaction. Journal of Clinical Pathology, v. 44, n. 1, p. 610–611, 1991. HARRUS, S.; MUMCUOGLU, K. Y.; MORICK, D.; EYAL, O.; BANETH, G. Molecular detection of Ehrlichia canis , Anaplasma bovis , Anaplasma platys , Candidatus Midichloria mitochondrii and Babesia canis vogeli in ticks from Israel. Clinical Microbiology and Infection, v. 17, n. 3, 2010. HARRUS, S.; PERLMAN-AVRAHAMI, A.; MUMCUOGLU, K. Y.; MORICK, D.; BANETH, G. Molecular detection of Rickettsia massiliae , Rickettsia sibirica mongolitimonae and Rickettsia conorii israelensis in ticks from Israel. Clinical Microbiology and Infection, v. 17, n. 2, p. 176–180, 2011. HARRUS, S.; WANER, T. Diagnosis of canine monocytotropic ehrlichiosis ( Ehrlichia canis): An overview. The Veterinary Journal, v. 187, n. 3, p. 292–296, 2011. HEBERT, P. D. N.; PENTON, E. H.; BURNS, J. M.; JANZEN, D. H.; HALLWACHS, W. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences, v. 101, n. 41, p. 14812–7, 2004. HILL, C. A.; GUTIERREZ, J. A. A method for extraction and analysis of high quality genomic DNA from ixodid ticks. Medical and Veterinary Entomology, v. 17, p. 224–227, 2003. HODGKINSON, J. E. Molecular diagnosis and equine parasitology. Veterinary Parasitology, v. 136, p. 109–116, 2006. HUBBARD, M. J.; CANN, K. J.; WRIGHT, D. J. M. Validation and rapid extraction of nucleic acids from alcohol-preserved ticks. Experimental and Applied Acarology, v. 19, p. 473–478, 1995. 42 INOKUMA, H.; BEPPU, T.; OKUDA, M.; SHIMADA, Y.; SAKATA, Y. Epidemiological survey of Anaplasma platys and Ehrlichia canis using ticks collected from dogs in Japan. Veterinary Parasitology, v. 115, p. 343–348, 2003. IRWIN, P. J. Canine babesiosis: from molecular taxonomy to control. Parasites & Vectors, v. 2, n. Suppl 1, p. S4, 2009. IRWIN, P. J. Canine Babesiosis. Veterinary Clinics of North America - Small Animal Practice, v. 40, n. 6, p. 1141–1156, 2010. JACOBS, D. E.; SCHNIEDER, T. Application of molecular biology to the diagnosis of parasitic disease. Veterinary Parasitology, v. 136, p. 67–68, 2006. JOHNSON, E. M.; EWING, S. A.; BARKER, R. W.; FOX, J. C.; CROW, D. W.; KOCAN, K. M. Experimental transmission of Ehrlichia canis (Rickettsiales : Ehrlichieae) by Dermacentor variabilis (Acari : Ixodidae). Veterinary Parasitology, v. 74, p. 277–288, 1998. KAMANI, J.; BANETH, G.; MUMCUOGLU, K. Y.; WAZIRI, N. E.; EYAL, O. Molecular detection and characterization of tick-borne pathogens in dogs and ticks from Nigeria. PLOS Neglected Tropical Diseases, v. 7, n. 3, p. 1–8, 2013. KHASNATINOV, M. A.; LIAPUNOV, A. V.; MANZAROVA, E. L.; KULAKOVA, N. V.; PETROVA, I. V.; DANCHINOVA, G. A. The diversity and prevalence of hard ticks attacking human hosts in Eastern Siberia (Russian Federation) with first description of invasion of non-endemic tick species. Parasitology Research, v. 115, n. 2, p. 501–510, 2016. KOBAYASHI, A.; IWASAKI, H. Human feet bitten by multiple brown dog ticks, Rhipicephalus sanguineus. IDCases, v. 9, n. April, p. 8, 2017. KOCH, H. G. Oviposition of the brown dog tick (Acari: Ixodidae) in the laboratory. Annals of the Entomological Society of America, v. 75, n. 5, p. 583–586, 1982. KOSTRO, K.; STOJECKI, K.; GRZYBEK, M.; TOMCZUK, K. Characteristics, immunologiccal events, and diagnostics of Babesia spp. infection, with emphasis on Babesia canis. Bulletin of the Veterinary Institute of Pulawy, v. 59, p. 495–504, 2015. LABRUNA, M. B.; CAMARGO, L. M. A.; TERRASSINI, F. A.; FERREIRA, F.; SCHUMAKER, T. T. S.; CAMARGO, E. P. Ticks (Acari: Ixodidae) from the state of Rondônia, western Amazon, Brazil. Systematic and Applied Acarology, v. 10, n. 1, p. 17– 32, 2005. LACHAUD, L.; MARCHERGUI-HAMMAMI, S.; CHABBERT, E.; DEREURE, J.; DEDET, J. P.; BASTIEN, P. Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis. Journal of Clinical Microbiology, v. 40, n. 1, p. 210–215, 2002. LEMOS, T. D. Frequência e caracterização molecular de Babesia spp . em cães naturalmente infectados no município de Teresópolis - RJ. 2009. Universidade Federal Fluminense. LITTLE, S. E.; HOSTETLER, J.; KOCAN, K. M. Movement of Rhipicephalus sanguineus 43 adults between co-housed dogs during active feeding. Veterinary Parasitology, v. 150, n. 1– 2, p. 139–145, 2007. LONG, S. W.; ZHANG, X.; ZHANG, J.; RUBLE, R. P.; TEEL, P.; YU, X. Evaluation of Transovarial transmission and transmissibility of Ehrlichia chaffeensis (Rickettsiales : Anaplasmataceae) in Amblyomma americanum (Acari : Ixodidae ). Journal of medical entomology, v. 40, n. 6, 2003. MAGNARELLI, L. A. Global Importance of Ticks and Associated Infectious Disease Agents. Clinical Microbiology Newsletter, v. 31, n. 5, p. 33–37, 2009. MANOEL, C. S. Alterações clínicas , hematológicas e sorológicas de cães infectados por Ehrlichia canis. 2010. Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo. MATEI, I. A.; D’AMICO, G.; YAO, P. K.; IONICĂ, A. M.; KANYARI, P. W. N.; DASKALAKI, A. A.; DUMITRACHE, M. O.; SÁNDOR, A. D.; GHERMAN, C. M.; QABLAN, M.; MODRÝ, D.; MIHALCA, A. D. Molecular detection of Anaplasma platys infection in free-roaming dogs and ticks from Kenya and Ivory Coast. Parasites & Vectors, v. 9, n. 1, p. 157, 2016. MEDEIROS-SILVA, V.; GURGEL-GONÇALVES, R.; NITZ, N.; MORALES, L. E. D. A.; CRUZ, L. M.; SOBRAL, I. G.; BOITÉ, M. C.; FERREIRA, G. E. M.; CUPOLILLO, E.; ROMERO, G. A. S. Successful isolation of Leishmania infantum from Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) collected from naturally infected dogs. BMC Veterinary Research, v. 11, n. 1, p. 258, 2015. MEHLHORN, H.; SCHEIN, E. The piroplasms: life cycle and sexual stages. Adv Parasitology, v. 23, p. 370–403, 1984. MELO, A. L. T.; WITTER, R.; MARTINS, T. F.; PACHECO, T. A.; ALVES, A. S.; CHITARRA, C. S.; DUTRA, V.; NAKAZATO, L.; PACHECO, R. C.; LABRUNA, M. B.; AGUIAR, D. M. A survey of tick-borne pathogens in dogs and their ticks in the Pantanal biome, Brazil. Medical and Veterinary Entomology, v. 30, n. 1, p. 112–116, 2016. MENTZ, M. B.; TROMBKA, M.; SILVA, G. L. da; SILVA, C. E. Rhipicephalus sanguineus (ACARI: IXODIDAE) biting a human being in Porto Alegre city, Rio Grande do Sul, Brazil. Revista do Instituto de Medicina Tropical de São Paulo, v. 58, n. 35, p. 2–4, 2016. MONTEIRO, S. G. Parasitologia na Medicina Veterinária. 1. ed. São Paulo: ROCA, 2011. MONTERO-PAU, J.; GÓMEZ, A.; MUÑOZ, J. Application of an inexpensive and highthroughput genomic DNA extraction method for the molecular ecology of zooplanktonic diapausing eggs. Limnology and Oceanography: Methods, v. 6, p. 218–222, 2008b. MORAES-FILHO, J.; KRAWCZAK, F. S.; COSTA, F. B.; SOARES, J. F.; LABRUNA, M. B. Comparative evaluation of the vector competence of four south american populations of the Rhipicephalus sanguineus group for the bacterium Ehrlichia canis , the agent of canine monocytic ehrlichiosis. PLOS ONE, p. 1–17, 2015. MYLONAKIS, M. E.; KOUTINAS, A. F.; BILLINIS, C.; LEONTIDES, L. S.; KONTOS, 44 V.; PAPADOPOULOS, O.; RALLIS, T.; FYTIANOU, A. Evaluation of cytology in the diagnosis of acute canine monocytic ehrlichiosis (Ehrlichia canis): A comparison between five methods. Veterinary Microbiology, v. 91, n. 2–3, p. 197–204, 2003. NAKAGHI, A. C. H. Clonagem do gene p28 e análise da expressão da proteína recombinante a partir da amostra Jaboticabal de Ehrlichia canis e sua aplicação no diagnóstico da erliquiose canina. 2008. Universidade Estadual Paulista. NAZARI, M.; LIM, S. Y.; WATANABE, M.; SHARMA, R. S. K.; CHENG, N. A. B. Y.; WATANABE, M. Molecular detection of Ehrlichia canis in dogs in Malaysia. PLoS neglected tropical diseases, v. 7, n. 1, 2013. NICHOLSON, W. L.; PADDOCK, C. D.; DEMMA, L.; TRAEGER, M.; JOHNSON, B.; DICKSON, J.; MCQUISTON, J.; SWERDLOW, D. Rocky mountain spotted fever in Arizona : Documentation of heavy environmental infestations of Rhipicephalus sanguineus at an endemic site. Annals of the New York Academy of Sciences, p. 338–341, 2006. NÓBREGA, K. Q. Estudo das principais doenças infecciosas em cães atendidos no Hospital Veterinário da Universidade de Brasília entre 2011 e 2014. 2015. Universidade de Brasília. O’CONNOR, T. P.; HANSCOM, J. L.; HEGARTY, B. C.; GROAT, R. G.; BREITSCHWERDT, E. B. Comparison of an indirect immunofluorescence assay, western blot analysis, and a commercially available ELISA for detection of Ehrlichia canis antibodies in canine sera. American Journal of Veterinary Research, v. 67, n. 2, p. 206–210, 2006. O’DWYER, L. H. Diagnóstico de hemoparasitos e carrapatos de cães procedentes de áreas rurais em três mesorregiões do estado do Rio de Janeiro , Brasil. 2000. Universidade Federal Rural do Rio de Janeiro. O’DWYER, L. H.; LOPES, V. V. A.; RUBINI, A. S.; PADUAN, K. dos S.; RIBOLLA, P. E. M. Babesia spp . infection in dogs from rural areas of São Paulo State , Brazil. Revista Brasileira de Parasitologia Veterinária, v. 18, n. 2, p. 23–26, 2009. OIE. Quality management in veterinary testing laboratories. In: OIE Manual of diagnostic tests and vaccines for terrestrial animals. Paris: Office International des Epizooties (OIE), 2004. p. 1–31. OLIVEIRA, A. C. de. Diagnóstico das hemoparasitoses caninas por biologia molecular, alterações hematológicas e centrifugação por gradiente. 2015. Universidade Federal de Viçosa. OLMEDA, A. S.; ARMSTRONG, P. M.; ROSENTHAL, B. M.; VALLADARES, A.; CASTILHO, A. del; ARMAS, F. de; MIGUELEZ, M.; GONZÁLEZ, A.; RODRÍGUEZ, J. A.; SPIELMAN, A.; TELFORD III, S. R. Short report - A subtropical case of human babesiosis. Acta Tropica, v. 67, p. 229–234, 1997. OSKAM, C. L.; GOFTON, A. W.; GREAY, T. L.; YANG, R.; DOGGETT, S.; RYAN, U. M.; IRWIN, P. J. Molecular investigation into the presence of a Coxiella sp. in Rhipicephalus sanguineus ticks in Australia. Veterinary Microbiology, v. 201, p. 141–145, 2017. 45 OTRANTO, D.; TESTINI, G.; DANTAS-TORRES, F.; LATROFA, M. S.; DINIZ, P. P. V. de P.; CAPRARIIS, D. de; LIA, R. P.; MENCKE, N.; STANNECK, D.; CAPELLI, G.; BREITSCHWERDT, E. B. Diagnosis of canine vector-borne diseases in young dogs : a longitudinal study. Journal of Clinical Microbiology, v. 48, n. 9, p. 3316–3324, 2010. PACHECO, R. C.; MORAES-FILHO, J.; GUEDES, E.; SILVEIRA, I.; RICHTZENHAIN, L. J.; LEITE, R. C.; LABRUNA, M. B. Rickettsial infections of dogs, horses and ticks in Juiz de Fora, southeastern Brazil, and isolation of Rickettsia rickettsii from Rhipicephalus sanguineus ticks. Medical and Veterinary Entomology, v. 25, n. 2, p. 148–155, 2011. PAT-NAH, H.; RODRIGUEZ-VIVAS, R. I.; BOLIO-GONZALEZ, M. E.; VILLEGASPEREZ, S. L. U. Z.; REYES-NOVELO, E. Molecular diagnosis of Ehrlichia canis in dogs and ticks Rhipicephalus sanguineus ( Acari : Ixodidae ) in Yucatan , Mexico. Journal of Medical Entomology, v. 52, n. 1, 2015. PEEL, M. C.; FINLAYSON, B. L.; MCMAHON, T. A. World map of the Köppen-Geiger climate classification. Hydrology and Earth System Sciences, v. 11, p. 259–263, 2006. PELEG, O.; BANETH, G.; EYAL, O.; INBAR, J.; HARRUS, S. Multiplex real-time qPCR for the detection of Ehrlichia canis and Babesia canis vogeli. Veterinary Parasitology, v. 173, n. 3–4, p. 292–299, 2010. PEREZ, M.; BODOR, M.; ZHANG, C.; XIONG, Q.; RIKIHISA, Y. Human Infection with Ehrlichia canis accompanied by clinical signs in Venezuela. Annals of the New York Academy of Sciences, v. 1078, p. 110–117, 2006. PIRANDA, E. M.; FACCINI, J. L. H.; PINTER, A.; PACHECO, R. C.; CANÇADO, P. H. D.; LABRUNA, M. B. Experimental infection of Rhipicephalus sanguineus ticks with the bacterium Rickettsia rickettsii, using experimentally infected dogs. Vector borne and zoonotic diseases, v. 11, n. 1, p. 29–36, 2011. POST, R. J.; FLOOKT, P. K.; MILLEST, A. L. Methods for the preservation of insects for DNA studies. Biochemical Systematics and Ecology, v. 21, n. 1, p. 85–92, 1993. RAMOS, R.; RAMOS, C.; ARAÚJO, F.; OLIVEIRA, R.; SOUZA, I.; PIMENTEL, D.; GALINDO, M.; SANTANA, M.; ROSAS, E.; FAUSTINO, M.; ALVES, L. Molecular survey and genetic characterization of tick-borne pathogens in dogs in metropolitan Recife (northeastern Brazil). Parasitology Research, v. 107, p. 1115–1120, 2010. RODRÍGUEZ, I.; FRAGA, J.; NODA, A. A.; MAYET, M.; DUARTE, Y.; ECHEVARRIA, E.; FERNÁNDEZ, C. An alternative and rapid method for the extraction of nucleic acids from ixodid ticks by potassium acetate procedure. Brazilian Archives of Biology and Technology, v. 57, n. 4, p. 542–547, 2014. ROTONDANO, T. E. de F.; ALMEIDA, H. K.; KRAWCZAK, F. S.; SANTANA, V. L.; VIDAL, I. F.; LABRUNA, M. B.; AZEVEDO, S. S. de; ALMEIDA, A. M. P. de; MELO, M. A. de. Survey of Ehrlichia canis, Babesia spp. and Hepatozoon spp. in dogs from a semiarid region of Brazil. Brazilian Journal of Veterinary Parasitology, v. 24, n. 1, p. 52–58, 2015. RUDOLER, N.; BANETH, G.; EYAL, O.; STRATEN, M. Van; HARRUS, S. Evaluation of 46 an attenuated strain of Ehrlichia canis as a vaccine for canine monocytic ehrlichiosis. Vaccine, v. 31, p. 226–233, 2012. SAINZ, Á.; ROURA, X.; MIRÓ, G.; ESTRADA-PEÑA, A.; KOHN, B.; HARRUS, S. Guideline for veterinary practitioners on canine ehrlichiosis and anaplasmosis in Europe. Parasites & Vectors, v. 75, n. 8, p. 1–20, 2015. SALEM, N. Y.; FARAG, H. S. Clinical , Hematologic , and molecular findings in naturally occurring Babesia canis vogeli in egyptian dogs. Veterinary Medicine International, v. 2014, p. 16–19, 2014. SALGADO, F. P. Identificação de hemoparasitos e carrapatos de cães procedentes do centro de controle de zoonoses de campo grande estado do Mato Grosso do Sul, Brasil. 2006. Universidade Federal do Mato Grosso do Sul. SAMBROOK, J. Molecular cloning: a laboratory manual. 3rd. ed. NY: Harbor Laboratory Press, 2001. SAMBROOK, J.; FRITSCH, E. F.; MANIATIS, T. Molecular Cloning: A Laboratory Manual. 2. ed. [s.l.] Cold Spring Harbor Laboratory Press, 1989. SANTOLIN, Í. D. A. C. Estudo ecológico de carrapatos associados com aves no entorno do Tinguá, Nova Iguaçu, Rio de Janeiro, Brasil; seu papel como bioindicadores e/ou agentes patogênicos de risco para saúde silvestre e coletiva. 2014. Universidade Federal Rural do Rio de Janeiro. SANTOS, F.; COPPEDE, J. S.; PEREIRA, A. L. A.; OLIVEIRA, L. P.; ROBERTO, P. G.; BENEDETTI, R. B. R.; ZUCOLOTO, L. B.; LUCAS, F.; SOBREIRA, L.; MARINS, M. Molecular evaluation of the incidence of Ehrlichia canis , Anaplasma platys and Babesia spp. in dogs from Ribeirão Preto, Brazil. The Veterinary Journal, v. 179, p. 145–148, 2009. SANTOS, H. A.; THOMÉ, S. M. G.; BALDANI, C. D.; SILVA, C. B.; PEIXOTO, M. P.; PIRES, M. S.; VITARI, G. L. V; COSTA, R. L.; SANTOS, T. M.; ANGELO, I. C.; SANTOS, L. A.; FACCINI, J. L. H.; MASSARD, C. L. Molecular epidemiology of the emerging zoonosis agent Anaplasma phagocytophilum (Foggie, 1949) in dogs and ixodid ticks in Brazil. Parasites & vectors, v. 6, n. 555042, p. 348, 2013. SCHOTTHOEFER, A. M.; MEECE, J. K.; IVACIC, L. C.; BERTZ, P. D.; ZHANG, K.; WEILER, T.; UPHOFF, T. S.; FRITSCHE, T. R. Comparison of a real-time PCR method with serology and blood smear analysis for diagnosis of human anaplasmosis: Importance of infection time course for optimal test utilization. Journal of Clinical Microbiology, v. 51, n. 7, p. 2147–2153, 2013. SHORTT, H. E. Babesia canis: The life cycle and laboratory maintenance in its arthropod and mammalian hosts. International Journal for Parasitology, v. 3, 1973. SILVEIRA, A. G.; PASSOS, L. M. F.; RIBEIRO, M. F. B. Population dynamics of Rhipicephalus sanguineus ( Latrielle , 1806 ) in Minas Gerais state, Brazil. Veterinary Parasitology, v. 161, p. 270–275, 2009. SMITH, F. D.; ELLSE, L.; WALL, R. Prevalence of Babesia and Anaplasma in ticks 47 infesting dogs in Great Britain. Veterinary Parasitology, v. 198, n. 1–2, p. 18–23, 2013. SOLANO-GALLEGO, L.; BANETH, G. Babesiosis in dogs and cats - Expanding parasitological and clinical spectra. Veterinary Parasitology, v. 181, n. 1, p. 48–60, 2011. SOLANO-GALLEGO, L.; RODRIGUEZ-CORTES, A.; TROTTA, M.; ZAMPIERON, C.; RAZIA, L.; FURLANELLO, T.; CALDIN, M.; ROURA, X.; ALBEROLA, J. Detection of Leishmania infantum DNA by fret-based real-time PCR in urine from dogs with natural clinical leishmaniosis. Veterinary Parasitology, v. 147, p. 315–319, 2007. SOLANO-GALLEGO, L.; TROTTA, M.; RAZIA, L.; FURLANELLO, T.; CALDIN, M. Molecular survey of Ehrlichia canis and Anaplasma phagocytophilum from blood of dogs in Italy. Annals of the New York Academy of Sciences, v. 1078, p. 515–518, 2006. SPARKES, J.; KORTNER, G.; BALLARD, G.; FLEMING, P. J. S.; BROWN, W. Y. Effects of sex and reproductive state on interactions between free-roaming domestic dogs. PLOS ONE, p. 1–14, 2014. SPOLIDORIO, M. G.; MINERVINO, A. H. H.; VALADAS, S. Y. O. B.; SOARES, H. S.; NEVES, A. L.; LABRUNA, M. B.; RIBEIRO, M. F. B.; GENNARI, S. M. Serosurvey for tick-borne diseases in dogs from the Eastern Amazon , Brazil. Revista Brasileira de Parasitologia Veterinária, v. 22, n. 2, p. 214–219, 2013. SPOLIDORIO, M. G.; TORRES, M. de M.; CAMPOS, W. N. da S.; MELO, A. L. T.; IGARASHI, M.; AMUDE, A. M.; LABRUNA, M. B.; AGUIAR, D. M. Molecular detection of Hepatozoon canis and Babesia canis vogeli in domestic dogs from Cuiabá , Brazil. Revista Brasileira de Parasitologia Veterinária, v. 20, n. 3, p. 253–255, 2011. TORINA, A.; BLANDA, V.; ANTOCI, F.; SCIMECA, S.; AGOSTINO, R. D.; SCARIANO, E.; PIAZZA, A.; GALLUZZO, P. A molecular survey of Anaplasma spp ., Rickettsia spp ., Ehrlichia canis and Babesia microti in foxes and fleas from Sicily. v. 60, p. 125–130, 2013. TRAPP, S. M.; MESSICK, J. B.; VIDOTTO, O.; JOJIMA, F. S.; AUTRAN, H. S.; MORAIS, D. Babesia gibsoni genotype Asia in dogs from Brazil. Veterinary Parasitology, v. 141, p. 177–180, 2006. TRUETT, G. E.; HEEGER, P.; MYNATT, R. L.; TRUETT, A. A.; WALKER, J. A.; WARMAN, M. L. Preparation of PCR-quality mouse genomic dna with hot sodium hydroxide and tris (HotSHOT). BioTechniques, v. 29, n. 1, p. 52–54, 2000. UENO, T. E. H.; AGUIAR, D. M.; PACHECO, R. C.; RICHTZENHAIN, L. J.; RIBEIRO, M. G.; PAES, A. C.; MEGID, J.; LABRUNA, M. B. Ehrlichia canis em cães atendidos em hospital veterinário de Botucatu , Estado de São Paulo , Brasil. Revista Brasileira de Parasitologia Veterinária, v. 18, n. 3, p. 57–61, 2009. VARELA, J. B. Influência dos métodos de conservação sobre a recuperação e a frequência de amplificação de marcadores mitocondriais e nucleares de carrapatos das espécies Amblyomma parvum e Amblyomma sculptum (Acari: Ixodidae). 2016. Universidade Federal Rural do Rio de Janeiro. VARGAS-HERNÁNDEZ, G.; ANDRÉ, M. R.; FARIA, J. L. M.; MUNHOZ, T. D.; 48 HERNANDEZ-RODRIGUEZ, M.; MACHADO, R. Z.; TINUCCI-COSTA, M. Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia. Veterinary Parasitology, v. 186, p. 254–260, 2012. VERLENGIA, R.; CURI, R.; BEVILACQUA, E.; NEWSHOLME, P. Análises de RNA, proteínas e metabólitos: metodologia e procedimentos técnicos. 1st. ed. São Paulo: Santos, 2013. VIEIRA, T. S. W. J.; VIEIRA, R. F. da C.; NASCIMENTO, D. A. G. do; TAMEKUNI, K.; TOLEDO, R. dos S.; CHANDRASHEKAR, R.; MARCONDES, M.; BIONDO, A. W.; VIDOTTO, O. Serosurvey of tick-borne pathogens in dogs from urban and rural areas from Parana state , Brazil. Revista Brasileira de Parasitologia Veterinária, v. 22, n. 1, p. 104– 109, 2013. VILELA, J. A. R.; PIRES, M. S.; SILVA, C. B. da; PEIXOTO, M. P.; FALQUETO, A.; SANTOS, H. A.; SANAVRIA, A.; FACCINI, J. L. H. Clinical-hematological changes due to Babesia canis vogeli infection in dogs from Seropédica , Rio de Janeiro , Brazil. REVISTA BRASILEIRA DE MEDICINA VETERINARIA, v. 35, n. 1, 2013. WIELINGA, P. R.; GAASENBEEK, C.; FONVILLE, M.; BOER, A. De; VRIES, A. De; DIMMERS, W.; AKKERHUIS, G.; JAGERS, O.; SCHOULS, L. M.; BORGSTEEDE, F.; GIESSEN, J. W. B. Van Der. Longitudinal analysis of tick densities and Borrelia , Anaplasma , and Ehrlichia infections of Ixodes ricinus ticks in different habitat areas in the netherlands. Applied and Environmental Microbiology, v. 72, n. 12, p. 7594–7601, 2006. WINKEL, K. T.; RIBEIRO, P. B.; ANTUNES, L. O.; CÁRCAMO, M. C.; VIANNA, É. E. S. Rhipicephalus sanguineus sensu lato (Ixodidae) in synantropic rodents in Rio Grande do Sul , Brazil. Brazilian Journal of Veterinary Parasitology, v. 23, n. 2, p. 276–279, 2014. WITTER, R.; VECCHI, S. N.; PACHECO, T. dos A.; MELO, A. L. T.; BORSA, A.; SINKOC, A. L.; MENDONÇA, A. J.; AGUIAR, D. M. Prevalência da erliquiose monocítica canina e anaplasmose trombocítica em cães suspeitos de hemoparasitose em Cuiabá , Mato Grosso. Semina: Ciências Agrárias, v. 34, n. 6, p. 3811–3822, 2013. YBAÑEZ, A. P.; PEREZ, Z. O.; GABOTERO, S. R.; YANDUG, R. T.; KOTARO, M.; YBA, A. P.; INOKUMA, H. First molecular detection of Ehrlichia canis and Anaplasma platys in ticks from dogs in Cebu , Philippines. Ticks and Tick-borne Diseases, v. 3, p. 287– 292, 2012. ZHOU, J.; JIA, H.; NISHIKAWA, Y.; FUJISAKI, K.; XUAN, X. Babesia gibsoni rhoptryassociated protein 1 and its potential use as a diagnostic antigen. Veterinary Parasitology, v. 145, p. 16–20, 2007por
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