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dc.contributor.author | Castro, Wladymir Mendes Carvalho de | - |
dc.date.accessioned | 2024-07-09T15:51:52Z | - |
dc.date.available | 2024-07-09T15:51:52Z | - |
dc.date.issued | 2022-08-02 | - |
dc.identifier.citation | CASTRO, Wladymir Mendes Carvalho. Avaliação in vitro do óleo essencial de Caesalpinia pulcherrima(L)Sw. contra estágios imaturos e adultos de Ctenocephalides felis felis. 2022. 79 f. Dissertação (Mestrado em Química) - Instituto de Química, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2022. | pt_BR |
dc.identifier.uri | https://rima.ufrrj.br/jspui/handle/20.500.14407/17609 | - |
dc.description.abstract | A interação entre humanos e animais tem aumentado cada vez mais, e com isso, eleva- se a preocupação em relação à saúde desses animais. Gatos e cachorros estão suscetíveis à infestação por carrapatos e pulgas, sendo o Rhipicephalus sanguineus e Ctenocephalides felis felis os mais comuns. A pulga do gato, conhecida como C. felis felis, é um inseto hematófago, sem asas que parasita os animais domésticos, que necessitam de alimentação diária. Deste modo, os animais sofrem constantemente com irritações cutâneas, anemias e dermatites alérgicas. Diante do desenvolvimento de resistência desses insetos frente ao tratamento com produtos químicos, os óleos essenciais mostram-se como alternativas promissoras, visto que já possuem atividade descrita contra alguns insetos. Sabendo-se desse potencial dos óleos essenciais, o presente trabalho tem como objetivo extrair o óleo essencial de C. pulcherrima, identificar seus componentes, avaliar sua atividade inseticida contra adultos e estágios imaturos da pulga do gato. Foram realizados testes in vitro de screening, para avaliar o comportamento das pulgas frente ao óleo essencial, em 10 concentrações diferentes no período de 24 e 48 horas utilizando-se 10 pulgas. Utilizou-se a técnica de impregnação em papel filtro. O óleo essencial de C. pulcherrima é rico em germacreno D (47,04 %) e apresentou atividade contra C. felis. Contra adultos, foi possível observar mortalidade de 15% e 40% a 800 μg cm-2 , no período de 24 e 48 horas, respectivamente. Contra ovos e pupas, observou-se 100% de mortalidade na concentração máxima (800 μg cm-2 ); já contra larvas, observou-se mortalidade máxima a 400 μg cm-2 em ambos os tempos testados. Testou-se também a atividade do óleo essencial, em duas concentrações, na inibição da enzima acetilcolinesterase, mas não foi possível observar esta inibição frente às duas concentrações analisadas. O óleo essencial estudado apresentou atividade significativa tanto para adultos quanto para os estágios imaturos de C. felis concluindo- se que possa ser um ótimo candidato para o controle de pulgas, sendo utilizado como uma alternativa para o desenvolvimento de pulicidas naturais. | pt_BR |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES | pt_BR |
dc.language | por | pt_BR |
dc.publisher | Universidade Federal Rural do Rio de Janeiro | pt_BR |
dc.subject | óleos essenciais | pt_BR |
dc.subject | pulicidas | pt_BR |
dc.subject | testes in vitro | pt_BR |
dc.subject | essential oils | pt_BR |
dc.subject | pulicidal | pt_BR |
dc.subject | in vitro tests | pt_BR |
dc.title | Avaliação in vitro do óleo essencial de caesalpinia pulcherrima (L.) Sw. contra estágios imaturos e adultos de ctenocephalides felis felis | pt_BR |
dc.title.alternative | In vitro evaluation of the essential oil of caesalpinia pulcherrima (L.) Sw. against immature and adult stages of ctenocephalides felisfelis | en |
dc.type | Dissertação | pt_BR |
dc.description.abstractOther | The interaction between humans and animals has increased more and more, and with that, the concern about the health of these animals increases. Cats and dogs are susceptible to tick and flea infestation, with Rhipicephalus sanguineus and Ctenocephalides felis felis being the most common. The cat flea, known as C. felis felis, is a hematophagous, wingless insect that parasitizes domestic animals that need daily food. In this way, animals are constantly suffering from skin irritations, anemia and allergic dermatitis. Given the development of resistance of these insects to treatment with chemical products, essential oils show themselves as promising alternatives, as they already have described activity against some insects. Knowing this potential of essential oils, the present work aims to extract the essential oil from Caesalpinia pulcherrima, identify its components, evaluate its insecticidal activity against adults and immature stages of cat flea. In vitro screening tests were carried out to evaluate the behavior of fleas against the essential oil, at 10 different concentrations in the period of 24 and 48 hours, using 10 fleas. The technique of impregnation on filter paper was used. The essential oil of C. pulcherrima is rich in germacrene D (47.04%) and has activity against C. felis. Against adults, it was possible to observe mortality between 15% and 40% at 800 μgcm-2 , in the period of 24 and 48 hours, respectively. Against eggs and pupae, 100% mortality was observed at the maximum concentration (800 μgcm-2 ); against larvae, maximum mortality was observed at 400 μgcm-2 in both times tested. The activity of the essential oil was also tested, in two concentrations, in the inhibition of the acetylcholinesterase enzyme, but it was not possible to observe this inhibition in the two analyzed concentrations. The essential oil studied showed significant activity for both adults and immature stages of C. felis, concluding that it may be a great candidate for flea control, being used as an alternative for the development of natural pulicidal. | en |
dc.contributor.advisor1 | Chaves, Douglas Siqueira de Almeida | - |
dc.contributor.advisor1Lattes | http://lattes.cnpq.br/1864237318361425 | pt_BR |
dc.contributor.referee1 | Chaves, Douglas Siqueira de Almeida | - |
dc.contributor.referee1Lattes | http://lattes.cnpq.br/1864237318361425 | pt_BR |
dc.contributor.referee2 | Salles, Cristiane Martins Cardoso de | - |
dc.contributor.referee2Lattes | http://lattes.cnpq.br/3610279707231709 | pt_BR |
dc.contributor.referee3 | Fingolo, Catharina Eccard | - |
dc.contributor.referee3Lattes | http://lattes.cnpq.br/3921610346020676 | pt_BR |
dc.creator.Lattes | http://lattes.cnpq.br/9230229346299288 | pt_BR |
dc.publisher.country | Brasil | pt_BR |
dc.publisher.department | Instituto de Química | pt_BR |
dc.publisher.initials | UFRRJ | pt_BR |
dc.publisher.program | Programa de Pós-Graduação em Química | pt_BR |
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