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dc.contributor.authorFreitas, Juliana Pereira de-
dc.date.accessioned2024-09-25T18:59:46Z-
dc.date.available2024-09-25T18:59:46Z-
dc.date.issued2022-02-24-
dc.identifier.citationFREITAS, Juliana Pereira de. Eficácia pulicida e residual in vitro do óleo essencial de Illicium verum contra Ctenocephalides felis felis (BOUCHÉ, 1835) (SIPHONAPTERA: PULICIDAE). 2022. 67 f. Dissertação (Mestrado em Ciências Veterinárias) - Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2022.pt_BR
dc.identifier.urihttps://rima.ufrrj.br/jspui/handle/20.500.14407/18246-
dc.description.abstractA pulga Ctenocephalides felis felis é o principal ectoparasita de cães e gatos, com relevância mundial. Em torno de 50% das patologias dermatológicas observadas nestes animais, estão relacionadas a presença de C. felis felis. Por conta de sua biologia, grandes infestações são frequentes, sendo muito comum o uso de inseticidas químicos para o controle das infestações. No entanto estes produtos apresentam algumas desvantagens relacionadas a degradação ambiental e resistência por parte dos organismos alvo. Este panorama tem sustentado as pesquisas em busca de compostos com atividade inseticida com potencial para o desenvolvivmento de produtos que possam representar alternativas aos utilizados atualmente, tornando crescente os estudos com inseticidas botânicos. O Illicium verum é uma planta medicinal da família Schisandracea, com frutos ricos em óleo essencial e com bioatividade contra vários insetos. O presente estudo teve como objetivo determinar a composição química do óleo essencial dos frutos de Illicium verum, avaliar sua atividade pulicida in vitro sobre pulgas adultas de Ctenocephalides felis felis bem como sua eficácia residual. Foi utilizado óleo essencial comercial. A análise química por cromatografia gasosa, permitiu identificar 16 compostos sendo que o (E)-anetol foi o constituinte majoritário representando 79,96% da composição. Utilizou-se o método de impregnação em tiras de papel filtro para a realização do teste in vitro. O óleo essencial de Illicium verum apresentou atividade inseticida contra adultos de Ctenocephalides felis felis, com 100% de eficácia na concentração de 800μg·cm-2 . Para a realização do teste de eficácia residual foi utilizado o método de papel filtro com a concentração que apresentou 100% de mortalidade, avaliou-se a mortalidade das pulgas a cada 24h, após cada avaliação as pulgas eram substituídas por pulgas novas advindas da mesma colônia, manteve-se as fitas impregnadas inicialmente, este procedimento foi repetido a cada 24 horas até não ser verificada mais eficácia pulicida. Foi observada bioatividade ao longo 18 dias.pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESpt_BR
dc.languageporpt_BR
dc.publisherUniversidade Federal Rural do Rio de Janeiropt_BR
dc.subjectÓleo essencialpt_BR
dc.subjectPulgaspt_BR
dc.subjectControlept_BR
dc.subjectEctoparasitaspt_BR
dc.titleEficácia Pulicida e Residual In Vitro do Óleo Essencial de Illicium verum Contra Ctenocephalides felis felis (Bouché, 1835) (Siphonaptera: Pulicidae)pt_BR
dc.title.alternativePulicidal efficacy and in vitro residue of the essential oil of Illicium verum against Ctenocephalides felis felis (BOUCHÉ, 1835) (SIPHONAPTERA: PULICIDAE)en
dc.typeDissertaçãopt_BR
dc.description.abstractOtherThe Ctenocephalides felis felis flea is the main ectoparasite of dogs and cats, transported worldwide. Around 50% of the dermatological pathologies observed in animals are related to the presence of C. felis felis. Due to its biology, large infestations are frequent, and the use of chemical insecticides to control infestations is very common. However, these products have disadvantages related to environmental degradation and resistance by target organisms. This panorama has supported research in search of compounds with insecticidal activity with potential for the development of products that may represent alternatives to those currently used, increasing studies with botanical insecticides. Illicium verum is a medicinal plant of the Schisandracea family, with fruits rich in essential oil and with bioactivity against several insects. The present study aimed to determine the chemical composition of the essential oil of Illicium verum fruits, evaluate its in vitro pulicidal activity on adult Ctenocephalides felis felis fleas as well as its residual efficacy. Commercial essential oil was used. Chemical analysis by gas chromatography identified 16 compounds, with (E)-anethole being the major constituent representing 79.96% of the composition. Use the paper filter strip impregnation method to perform the in vitro test. The essential oil of Illicium verum showed insecticidal activities against adults of Ctenocephalides felis felis, with 100% efficacy at a concentration of 800μg • cm-2. To carry out the residual efficacy test, the filter paper method was used with the concentration that presents 100% mortality, the mortality of the fleas was evaluated every 24 hours, after each evaluation how fleas were replaced by new fleas from the colony, kept as impregnated bulletin tapes, this procedure was repeated every 24 hours until no further purifying efficacy was verified. Bioactivity was observed over 18 days.en
dc.contributor.advisor1Chaves, Douglas Siqueira de Almeida-
dc.contributor.advisor1IDhttps://orcid.org/0000-0002-0571-9538pt_BR
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/1864237318361425pt_BR
dc.contributor.referee1Chaves, Douglas Siqueira de Almeida-
dc.contributor.referee1IDhttps://orcid.org/0000-0002-0571-9538pt_BR
dc.contributor.referee1Latteshttp://lattes.cnpq.br/1864237318361425pt_BR
dc.contributor.referee2Cid, Yara Peluso-
dc.contributor.referee2IDhttps://orcid.org/0000-0003-0775-0704pt_BR
dc.contributor.referee2Latteshttp://lattes.cnpq.br/0788912635109182pt_BR
dc.contributor.referee3Endringer, Denise Coutinho-
dc.contributor.referee3IDhttps://orcid.org/0000-0001-9396-2097pt_BR
dc.contributor.referee3Latteshttp://lattes.cnpq.br/6424432214565576pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/4154615014469392pt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Veterináriapt_BR
dc.publisher.initialsUFRRJpt_BR
dc.publisher.programPrograma de Pós-Graduação em Ciências Veterináriaspt_BR
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dc.subject.cnpqMedicina Veterináriapt_BR
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