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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Moreira, Leandra Oliveira | - |
| dc.date.accessioned | 2025-03-07T17:12:29Z | - |
| dc.date.available | 2025-03-07T17:12:29Z | - |
| dc.date.issued | 2022-03-31 | - |
| dc.identifier.citation | MOREIRA, Leandra Oliveira. Atividade in vitro do Óleo Essencial de Ocimum gratissimum L. sobre Ctenocephalides felis felis (Bouché, 1835) e Rhipicephalus sanguineus (Latreille, 1806). 2022. 76 f. Dissertação (Mestrado em Ciências Veterinárias, Biotecnologia e Inovação Tecnológica em Medicina Veterinária) - Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2022. | pt_BR |
| dc.identifier.uri | https://rima.ufrrj.br/jspui/handle/20.500.14407/20265 | - |
| dc.description.abstract | A pulga Ctenocephalides felis felis e o carrapato Rhipicephalus sanguineus são os principais ectoparasitas que acometem cães e gatos, por serem espécies presentes por todo o mundo, e com capacidade vetorial de uma série de doenças tanto para os animais, quanto para ao homem. O controle de infecções por ectoparasitas de importância veterinária ainda depende muito do uso de substâncias químicas que atinjam o sistema nervoso dos artrópodes. Tais compostos sofreram de uma série de inconvenientes, tais como o desenvolvimento de resistência e preocupações com a segurança do animal, humana e ambiental. Por isso, a busca por produtos e tecnologias mais seguras e eficazes tem sido um desafio para a indústria, é nesse contexto, que os estudos e o desenvolvimento de produtos de origens naturais ganham relevância. Na área na medicina veterinária, temos estudos no controle de carrapatos e pulgas com o óleo essencial de Ocimum sp que exibem uma gama de atividades biológicas como inseticida, acaricida e repelência de insetos. Diante disso, esse trabalho teve como objetivo avaliar o potencial do óleo essencial de Ocimum gratissimum L. no controle de formas imaturas e adultos da pulga C. felis felis e do carrapato R. sanguineus. Dentre os objetivos específicos estão a obtenção e caracterização química do óleo essencial das folhas de Ocimum gratissimum L., a avaliação da atividade in vitro em diferentes fases do ciclo da pulga C. felis felis (ovo, larva, pupa e adultos) e do carrapato R. sanguineus (larva, ninfa e adultos), e a determinação através da análise de probito da CL50 e a CL90 do óleo essencial das folhas de Ocimum gratissimum L. em diferentes fases do ciclo da pulga C. felis felis e do carrapato R. sanguineus. Observamos que o óleo essencial de Ocimum gratissimum L. apresentou 100% de mortalidade em todos os estágios analisados da pulga. Para o carrapato podemos observamos que na fase de larva atingimos 100% de mortalidade, enquanto que o máximo atingido de mortalidade para ninfas foi de 80,8%, e na fase adulta não atingimos mortalidade. A análise da composição química do óleo essencial de O. gratissimum mostrou que os principais compostos encontrados foram Eugenol (77,7%) e Pineno (5,26%). Determinamos através da análise de probito as CL50 e a CL90 a seguir: ovo (CL50 = 10,54; CL90 = 22,00), larva 24h (CL50 = 21,06; CL90 = 59,79), larva 48h (CL50 = 13,20; CL90 = 38,56), pupa (CL50 = 18,69; CL90 = 69,26), adulto 24h (CL50 = 20,24; CL90 = 55,90), adulto 48h (CL50 = 15,72; CL90 = 44,72) e ID (CL50 = 2,00; CL90 = 5,18). Foi possível obter, caracterizar, avaliar o potencial inseticida e acaricida e determinar através da análise de probito a CL50 e CL90 do óleo essencial de Ocimum gratissimum L. em todas as fases do ciclo da pulga C. felis felis (ovo, larva, pupa e adultos) e do carrapato R. sanguineus (larva, ninfa e adultos). | pt_BR |
| dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES | pt_BR |
| dc.language | por | pt_BR |
| dc.publisher | Universidade Federal Rural do Rio de Janeiro | pt_BR |
| dc.subject | Ocimum gratissimum L | pt_BR |
| dc.subject | Ectoparasitas | pt_BR |
| dc.subject | Inseticida | pt_BR |
| dc.subject | Ectoparasites | pt_BR |
| dc.subject | Insecticide | pt_BR |
| dc.title | Atividade in vitro do Óleo Essencial de Ocimum gratissimum L. sobre Ctenocephalides felis felis (Bouché, 1835) e Rhipicephalus sanguineus (Latreille, 1806) | pt_BR |
| dc.title.alternative | In vitro activity of Ocimum gratissimum L. Essential Oil on Ctenocephalides felis felis (Bouché, 1835) and Rhipicephalus sanguineus (Latreille, 1806). | en |
| dc.type | Dissertação | pt_BR |
| dc.description.abstractOther | The flea Ctenocephalides felis felis and the tick Rhipicephalus sanguineus are the main ectoparasites that affect dogs and cats, as they are species present all over the world, and with vector capacity of a series of diseases for both animals and humans. The control of infections by ectoparasites of veterinary importance still depends a lot on the use of chemical substances that reach the nervous system of arthropods. Such compounds have suffered from a number of drawbacks, such as the development of resistance and concerns for animal, human and environmental safety. Therefore, the search for safer and more effective products and technologies has been a challenge for the industry, it is in this context that the studies and development of products from natural origins gain relevance. In the area of veterinary medicine, we have studies in the control of ticks and fleas with the essential oil of Ocimum sp that exhibit a range of biological activities such as insecticide, acaricide and insect repellency. Therefore, this work aimed to evaluate the potential of the essential oil of Ocimum gratissimum L. in the control of immature and adult forms of the flea Ctenocephalides felis felis and the tick R. sanguineus. Among the specific objectives are the obtaining and chemical characterization of the essential oil from the leaves of Ocimum gratissimum L., the evaluation of the in vitro activity in different phases of the cycle of the flea C. felis felis (egg, larva, pupa and adults) and the tick R. sanguineus (larva, nymph and adults), and the determination through probit analysis of the LC50 and LC90 of the essential oil of the leaves of Ocimum gratissimum L. in different phases of the cycle of the flea Ctenocephalides felis felis and the tick R. sanguineus. We observed that the essential oil of Ocimum gratissimum L. presented 100% mortality in all analyzed flea stages. For the tick we can observe that in the larval stage we reached 100% mortality, while the maximum mortality reached for nymphs was 80.8%, and in the adult stage we did not reach mortality. The analysis of the chemical composition of the essential oil of O. gratissimum showed that the main compounds found were Eugenol (77.7%) and Pinene (5.26%). We determined by probit analysis the following LC50 and LC90: egg (LC50 = 10.54; LC90 = 22.00), larva 24h (LC50 = 21.06; LC90 = 59.79), larva 48h (LC50 = 13.20; LC90 = 38.56), pupa (LC50 = 18.69; LC90 = 69.26), adult 24h (LC50 = 20.24; LC90 = 55.90), adult 48h (LC50 = 15.72; LC90 = 44.72) and ID (CL50 = 2.00; CL90 = 5.18). It was possible to obtain, characterize, evaluate the insecticidal and acaricidal potential and determine through probitus analysis the LC50 and LC90 of the essential oil of Ocimum gratissimum L. in all phases of the flea cycle C. felis felis (egg, larva, pupa and adults) and the tick R. sanguineus (larva, nymph and adults). | en |
| dc.contributor.advisor1 | Cid, Yara Peluso | - |
| dc.contributor.advisor1ID | https://orcid.org/0000-0003-0775-0704 | pt_BR |
| dc.contributor.advisor1Lattes | http://lattes.cnpq.br/0788912635109182 | pt_BR |
| dc.contributor.advisor-co1 | Chaves, Douglas Siqueira de Almeida | - |
| dc.contributor.advisor-co1ID | https://orcid.org/0000-0002-0571-9538 | pt_BR |
| dc.contributor.advisor-co1Lattes | http://lattes.cnpq.br/1864237318361425 | pt_BR |
| dc.contributor.referee1 | Cid, Yara Peluso | - |
| dc.contributor.referee1ID | https://orcid.org/0000-0003-0775-0704 | pt_BR |
| dc.contributor.referee1Lattes | http://lattes.cnpq.br/0788912635109182 | pt_BR |
| dc.contributor.referee2 | Campos, Diefrey Ribeiro | - |
| dc.contributor.referee2ID | https://orcid.org/0000-0002-5434-1463 | pt_BR |
| dc.contributor.referee2Lattes | http://lattes.cnpq.br/8547992443497955 | pt_BR |
| dc.contributor.referee3 | Epifanio, Neide Mara de Menezes | - |
| dc.contributor.referee3ID | https://orcid.org/0000-0003-2842-6647 | pt_BR |
| dc.contributor.referee3Lattes | http://lattes.cnpq.br/6181150261358627 | pt_BR |
| dc.creator.Lattes | http://lattes.cnpq.br/3425394385627928 | pt_BR |
| dc.publisher.country | Brasil | pt_BR |
| dc.publisher.department | Instituto de Veterinária | pt_BR |
| dc.publisher.initials | UFRRJ | pt_BR |
| dc.publisher.program | Programa de Pós-Graduação em Ciências Veterinárias | pt_BR |
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The complete mitochondrial genome sequences of the cat flea Ctenocephalides felis felis (Siphonaptera: Pulicidae) support the hypothesis that C. felis isolates from China and USA were the same C. f. felis subspecies. Acta Tropica, v. 217, 2021. ZHAO, X., YEH, J. Z., SALGADO, V. L., NARAHASHI, T. Fipronil is a potent open channel blocker of glutamate-activated chloride channels in cockroach 63 neurons. Journal of Pharmacology and Experimental Therapeutics, v. 310, n. 1, p. 192-201, 2004. | pt_BR |
| dc.subject.cnpq | Medicina Veterinária | pt_BR |
| Appears in Collections: | Mestrado em Ciências Veterinárias | |
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