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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Rabello, Carla Alves | - |
| dc.date.accessioned | 2025-06-10T12:07:51Z | - |
| dc.date.available | 2025-06-10T12:07:51Z | - |
| dc.date.issued | 2025-02-28 | - |
| dc.identifier.citation | RABELLO, Carla Alves. Expressão diferencial de genes de vias imunológicas envolvidas na resposta de Rhipicephalus microplus (Acari: Ixodidae) à infecção por Theileria equi (Piroplasmida: Theileridae). 2025. 116 f. Dissertação (Mestrado em Ciências Veterinárias) - Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2025. | pt_BR |
| dc.identifier.uri | https://rima.ufrrj.br/jspui/handle/20.500.14407/22167 | - |
| dc.description.abstract | Theileria equi é um protozoário intraeritrocítico causador da piroplasmose equina, uma doença que acomete equídeos e é responsável por gerar prejuízos à saúde e à economia do setor equestre mundialmente. No Brasil, Rhipicephalus microplus é a única espécie de carrapato com capacidade de atuar como vetor de T. equi em condições experimentais. Contudo, os mecanismos envolvidos nesta interface patógeno-vetor ainda não foram completamente esclarecidos. Assim, o objetivo deste trabalho foi analisar a expressão diferencial de genes envolvidos no metabolismo redox e nas principais vias de sinalização imunológica de R. microplus em resposta à infecção por T. equi. Para isso, um equino portador crônico de T. equi (controle positivo) e um equino não infectado (controle negativo) foram infestados com larvas de R. microplus livres de patógenos. Após o período de fixação e repasto sanguíneo, larvas e ninfas inteiras foram coletadas e armazenadas individualmente, enquanto as fêmeas foram dissecadas e o intestino e a glândula salivar de cada exemplar foi armazenado de forma individual. Em seguida, foi feita a extração do RNA e a síntese do cDNA de cada amostra, os quais foram submetidos ao ensaio de expressão gênica relativa através da qPCR. No primeiro capítulo, foram analisados genes relacionados ao metabolismo redox de R. microplus em resposta à infecção por T. equi. Assim, observou-se uma regulação positiva tanto de genes pró- oxidantes quanto de genes antioxidantes no intestino e na glândula salivar de fêmeas ingurgitadas infectadas por T. equi, com destaque para os genes que codificam para as enzimas dual oxidases (pró-oxidante) e catalase (antioxidante), sendo ambos mais expressos no grupo infectado. Na glândula salivar, porém, o gene que codifica para a enzima glutationa-s- transferase foi significativamente suprimido no grupo infectado, sugerindo que tal enzima pode ter papel fundamental na resposta antioxidante neste tecido. Nos estágios de larva e ninfa não foi observada uma resposta oxidativa significativa. No segundo capítulo, foram analisados genes envolvidos nas vias de sinalização imunológica Toll, IMD e JAK/STAT, além de genes que codificam para os peptídeos antimicrobianos microplusina, defensina e ixodidina. A infecção por T. equi estimulou a ativação das vias Toll e IMD no intestino e na glândula salivar de R. microplus, resultando em uma regulação positiva dos genes microplusina e defensina em ambos os tecidos. A via JAK/STAT, contudo, foi modulada negativamente no intestino em resposta à infecção por T. equi, o que pode ter ocasionado a expressão diferencial não significativa do gene ixodidina observada neste tecido. Nos estágios de larva e ninfa não foi observada uma resposta significativa à infecção por T. equi através das vias analisadas. Os resultados obtidos neste estudo permitem elucidar os mecanismos moleculares envolvidos na interação entre T. equi e R. microplus, contribuindo para uma melhor compreensão acerca de como o carrapato responde à infecção pelo protozoário. Tais informações auxiliam na identificação de moléculas-chave com potencial para serem utilizadas no desenvolvimento de novas estratégias de prevenção e controle da piroplasmose equina no país. | pt_BR |
| dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES | pt_BR |
| dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro - FAPERJ | pt_BR |
| dc.language | por | pt_BR |
| dc.publisher | Universidade Federal Rural do Rio de Janeiro | pt_BR |
| dc.subject | Piroplasmose equina | pt_BR |
| dc.subject | Rhipicephalus microplus | pt_BR |
| dc.subject | interação patógeno-vetor | pt_BR |
| dc.subject | Equine piroplasmosis | pt_BR |
| dc.subject | pathogen-vector interface | pt_BR |
| dc.title | Expressão diferencial de genes de vias imunológicas envolvidas na resposta de Rhipicephalus microplus (Acari: Ixodidae) à infecção por Theileria equi (Piroplasmida: Theileridae) | pt_BR |
| dc.title.alternative | Differential expression of immune pathways genes in the tick Rhipicephalus microplus (Acari: Ixodidae) in response to Theileria equi (Piroplasmida: Theileridae) infection. | en |
| dc.type | Dissertação | pt_BR |
| dc.description.abstractOther | Theileria equi is an intraerythrocytic protozoan that causes equine piroplasmosis, a disease that affects equids and is responsible for significant health and economic losses to the global equine industry. In Brazil, Rhipicephalus microplus is the only tick species experimentally proven to act as a vector for T. equi. However, the mechanisms involved in this pathogen-vector interaction have not yet been fully elucidated. Therefore, the aim of this study was to analyze the differential expression of genes involved in redox metabolism and the main immune signaling pathways of R. microplus in response to T. equi infection. For this, a chronically infected equine with T. equi (positive control) and a non-infected equine (negative control) were infested with pathogen-free larvae of R. microplus. After the fixation and blood feeding period, whole larvae and nymphs were collected and stored individually, while the females were dissected, and the midgut, ovary, and salivary gland of each specimen were stored separately. RNA was then extracted, and cDNA was synthesized from each sample, followed by relative gene expression assays. In the first chapter, genes related to the redox metabolism of R. microplus in response to T. equi infection were analyzed. A upregulation of both pro-oxidant and antioxidant genes was observed in the midgut and salivary gland of engorged females infected by T. equi, with a particular emphasis on the genes encoding the enzymes dual oxidases (pro-oxidant) and catalase (antioxidant), both being more expressed in the infected group. However, in the salivary gland, the gene encoding the enzyme glutathione-S-transferase was significantly suppressed in the infected group, suggesting that this enzyme may play a key role in the antioxidant response in this tissue. No significant oxidative response was observed in the larval and nymph stages, nor in the ovaries of engorged females. In the second chapter, genes involved in the Toll, IMD, and JAK/STAT immune signaling pathways, as well as genes encoding the antimicrobial peptides microplusin, defensin, and ixodidin, were analyzed. T. equi infection stimulated the activation of the Toll and IMD pathways in the midgut and salivary gland of R. microplus, resulting in a positive regulation of the microplusin and defensin genes in both tissues. However, the JAK/STAT pathway was suppressed in the midgut in response to T. equi infection, which may have led to the non-significant differential expression of the ixodidin gene observed in this tissue. No significant response to T. equi infection through the analyzed pathways was observed in the larval and nymph stages, nor in the ovaries of engorged females. The results obtained in this study provide insights into the molecular mechanisms involved in the interaction between T. equi and R. microplus, contributing to a better understanding of how the tick responds to protozoan infection. Such information aids in identifying key molecules with potential to be used in the development of new strategies for the prevention and control of equine piroplasmosis in the country. | en |
| dc.contributor.advisor1 | Santos, Huarrisson Azevedo | - |
| dc.contributor.advisor1ID | https://orcid.org/0000-0002-8218-3626 | pt_BR |
| dc.contributor.advisor1Lattes | http://lattes.cnpq.br/3751609492049306 | pt_BR |
| dc.contributor.advisor-co1 | Paulino, Patrícia Gonzaga | - |
| dc.contributor.advisor-co1ID | https://orcid.org/0000-0001-9326-7609 | pt_BR |
| dc.contributor.advisor-co1Lattes | http://lattes.cnpq.br/5946161927068059 | pt_BR |
| dc.contributor.referee1 | Santos, Huarrisson Azevedo | - |
| dc.contributor.referee1ID | https://orcid.org/0000-0002-8218-3626 | pt_BR |
| dc.contributor.referee1Lattes | http://lattes.cnpq.br/3751609492049306 | pt_BR |
| dc.contributor.referee2 | Gôlo, Patrícia Silva | - |
| dc.contributor.referee2ID | https://orcid.org/0000-0003-1854-7488 | pt_BR |
| dc.contributor.referee2Lattes | http://lattes.cnpq.br/3935275742919097 | pt_BR |
| dc.contributor.referee3 | Godinho, Rodrigo Maciel da Costa | - |
| dc.contributor.referee3Lattes | http://lattes.cnpq.br/5593991918808836 | pt_BR |
| dc.creator.Lattes | http://lattes.cnpq.br/4749180497231290 | pt_BR |
| dc.publisher.country | Brasil | pt_BR |
| dc.publisher.department | Instituto de Veterinária | pt_BR |
| dc.publisher.initials | UFRRJ | pt_BR |
| dc.publisher.program | Programa de Pós-Graduação em Ciências Veterinárias | pt_BR |
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| dc.subject.cnpq | Parasitologia | pt_BR |
| Appears in Collections: | Mestrado em Ciências Veterinárias | |
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