Caracterização molecular e toxicológica de Ctenocephalides felis felis Frente ao Fipronil

Barros, Guilherme Mota Maciel do Rêgo

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Campo DC	Valor	Idioma
dc.contributor.author	Barros, Guilherme Mota Maciel do Rêgo	-
dc.date.accessioned	2025-10-06T20:45:25Z	-
dc.date.available	2025-10-06T20:45:25Z	-
dc.date.issued	2025-02-25	-
dc.identifier.citation	BARROS, Guilherme Mota Macieldo do Rêgo. Caracterização molecular e toxicológica de Ctenocephalides felis felis frente ao Fipronil. 2025. 47 f. Dissertação (Ciências Veterinárias) - Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, 2025.	pt_BR
dc.identifier.uri	https://rima.ufrrj.br/jspui/handle/20.500.14407/23438	-
dc.description.abstract	A pulga é um importante ectoparasita que pode causar enfermidades em animais domésticos. Ctenocephalides felis felis é a espécie mais comum nesses animais e o Fipronil, um fenilpirazol, atua nos canais de cloro ativados pelo GABA (ácido gama-aminobutírico), levando à morte da pulga por hiperexcitação. Diante da possibilidade de resistência ao Fipronil em C. felis felis, é necessário monitorar essa questão. Assim, o objetivo deste estudo foi caracterizar, por meio de bioensaios, a susceptibilidade de C. felis felis ao Fipronil em uma cepa laboratorial. Além disso, buscou-se detectar a possível presença de mutações no gene Rdl (Resistance to Dieldrin) na mesma cepa laboratorial e em uma cepa de campo. O estudo também investigou a presença de Rickettsia felis, uma bactéria de importância veterinária, cujo principal hospedeiro é C. felis felis. Para a cepa laboratorial, foram determinados os valores de concentração letal (CL) para C. felis felis, sendo a CL50 de 10,39 ppm e a CL90 de 23,71 ppm. Nas análises moleculares, verificou-se que 94,73% (36/38) das pulgas da cepa laboratorial apresentavam homozigose para a mutação de resistência no gene Rdl. Na cepa de campo, esse mesmo genótipo foi encontrado em 90,83% (109/120) das pulgas. Além disso, 5% (6/120) das pulgas da cepa de campo apresentaram heterozigose para a mutação, enquanto 4,16% (5/120) possuíam homozigose para alelos sem a mutação associada à resistência. A presença de DNA de R. felis foi detectada em 94,73% (36/38) das pulgas da cepa laboratorial e em 27,50% (33/120) das pulgas da cepa de campo. Em comparação com outros estudos toxicológicos, a colônia laboratorial foi caracterizada como susceptível ao Fipronil, apesar da predominância do genótipo de resistência para o gene Rdl. Na cepa de campo, também houve predomínio desse genótipo. Com base nesses resultados, conclui-se que a mutação no gene Rdl, isoladamente, não é suficiente para conferir resistência ao Fipronil em C. felis felis. Neste estudo, a presença de R. felis pode ter influenciado a susceptibilidade das pulgas ao inseticida. Além disso, verificou-se que essa mutação é predominante tanto em populações laboratoriais quanto em populações de campo	pt_BR
dc.description.sponsorship	Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq	pt_BR
dc.language	por	pt_BR
dc.publisher	Universidade Federal Rural do Rio de Janeiro	pt_BR
dc.subject	Resistência parasitária	pt_BR
dc.subject	Ectoparasitos	pt_BR
dc.subject	Fenilpirazol	pt_BR
dc.subject	Endossimbionte	pt_BR
dc.subject	parasitic resistance	pt_BR
dc.subject	ectoparasites	pt_BR
dc.subject	phenylpyrazole	pt_BR
dc.subject	endosymbiont	pt_BR
dc.title	Caracterização molecular e toxicológica de Ctenocephalides felis felis Frente ao Fipronil	pt_BR
dc.title.alternative	Molecular and Toxicological Characterization from Ctenocephalides felis felis to Fipronil	en
dc.type	Dissertação	pt_BR
dc.description.abstractOther	The flea is an important ectoparasite that can cause diseases in domestic animals. Ctenocephalides felis felis is the most common species found in these animals, and Fipronil, a phenylpyrazole, acts on chloride channels activated by GABA (gamma-aminobutyric acid), leading to flea death through hyperexcitation. Given the potential for Fipronil resistance in C. felis felis, it is necessary to monitor this issue. Thus, the objective of this study was to characterize, through bioassays, the susceptibility of C. felis felis to Fipronil in a laboratory strain. Additionally, the study aimed to detect the possible presence of mutations in the Rdl (Resistance to Dieldrin) gene in both a laboratory strain and a field strain. Another objective was to investigate the presence of Rickettsia felis, an important bacterium for veterinary medicine, whose primary host is C. felis felis. For the laboratory strain, lethal concentration (LC) values were determined for C. felis felis, with LC50 at 10.39 ppm and LC90 at 23.71 ppm. Molecular analyses revealed that 94.73% (36/38) of the fleas from the laboratory strain were homozygous for the resistance mutation in the Rdl gene. In the field strain, the same genotype was found in 90.83% (109/120) of the fleas. Furthermore, 5% (6/120) of the fleas from the field strain were heterozygous for the mutation, while 4.16% (5/120) were homozygous for alleles without the resistance-associated mutation. The presence of R. felis DNA was detected in 94.73% (36/38) of the fleas from the laboratory strain and in 27,50% (33/120) of the fleas from the field strain. Compared to other toxicological studies, the laboratory colony was characterized as susceptible to Fipronil, despite the predominance of the resistance genotype in the Rdl gene. In the field strain, this genotype was also predominant. Based on these results, it can be concluded that the Rdl gene mutation alone is not sufficient to confer resistance to Fipronil in C. felis felis. In this study, the presence of R. felis may have played a role in the susceptibility of C. felis felis to the insecticide. Additionally, it was observed that this mutation is predominant in both laboratory and field flea populations	en
dc.contributor.advisor1	Azevedo, Thaís Ribeiro Correia	-
dc.contributor.advisor1ID	http://lattes.cnpq.br/6049103053269712	pt_BR
dc.contributor.advisor1Lattes	http://lattes.cnpq.br/6049103053269712	pt_BR
dc.contributor.referee1	Azevedo, Thaís Ribeiro Correia	-
dc.contributor.referee1ID	http://lattes.cnpq.br/6049103053269712	pt_BR
dc.contributor.referee1Lattes	http://lattes.cnpq.br/6049103053269712	pt_BR
dc.contributor.referee2	Furtado, Tassia Torres	-
dc.contributor.referee2ID	https://orcid.org/0000-0003-4439-0218	pt_BR
dc.contributor.referee2Lattes	http://lattes.cnpq.br/1503080262726213	pt_BR
dc.contributor.referee3	Doyle, Rovaina Laureano	-
dc.contributor.referee3ID	https://orcid.org/0000-0002-8282-6564	pt_BR
dc.contributor.referee3Lattes	http://lattes.cnpq.br/2961444018721919	pt_BR
dc.creator.ID	https://orcid.org/0000-0003-0753-0840	pt_BR
dc.creator.Lattes	http://lattes.cnpq.br/0821350616370628	pt_BR
dc.publisher.country	Brasil	pt_BR
dc.publisher.department	Instituto de Veterinária	pt_BR
dc.publisher.initials	UFRRJ	pt_BR
dc.publisher.program	Programa de Pós-Graduação em Ciências Veterinárias	pt_BR
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Insecticide resistance and management strategies in urban ecosystems. Insects, v. 7, n. 1, p. 2, 2016.	pt_BR
dc.subject.cnpq	Medicina Veterinária	pt_BR
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