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dc.contributor.authorMoraes, Lais de Jesus
dc.date.accessioned2023-12-21T18:42:50Z-
dc.date.available2023-12-21T18:42:50Z-
dc.date.issued2021-05-21
dc.identifier.citationMORAES, Lais de Jesus. Avaliação do prick test como um método auxiliar de diagnóstico de dermatite alérgica a picada de pulga em cães. 2021. 34 f. Dissertação (Mestrado em Ciências Veterinárias) - Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2021.por
dc.identifier.urihttps://rima.ufrrj.br/jspui/handle/20.500.14407/9700-
dc.description.abstractA dermatite alérgica a picada de pulgas (DAPP) é uma doença dermatológica comum no Brasil, que traz desconforto tanto para o animal, quanto ao tutor. O diagnóstico normalmente é feito através da anamnese, exame físico e resposta à terapia. Esses métodos são questionados pelo proprietário, hoje mais esclarecido e exigente, que busca um diagnóstico conclusivo, objetivo e rápido. Alguns estudos foram feitos com o teste intradérmico (TID) para auxiliar o diagnóstico dessa doença, porém os resultados apresentaram divergências. Uma alternativa seria a utilização do prick test (PT), que tem sido amplamente aceito na medicina humana como um método rápido, seguro, confiável e de baixo custo. O objetivo do estudo foi avaliar o prick test como método auxiliar no diagnóstico de dermatite alérgica à picada de pulga em cães. Foram selecionados 24 cães do Laboratório de Quimioterapia Experimental em Parasitologia Veterinária (LQEPV) da raça beagle, machos e fêmeas, com idade igual ou superior a um ano. Esses animais foram alocados em dois grupos: o grupo 1 contendo 12 beagles sabidamente sem DAPP e o grupo 2 composto por 12 cães com diagnóstico clínico de DAPP. Os animais foram contidos fisicamente, colocados em decúbito lateral e tiveram a região do tórax tricotomizada e higienizada com solução fisiológica. Foi utilizada uma gota de cada solução: para o controle negativo foi utilizada uma solução salina glicerinada (50% de glicerol - estabilizante de proteínas) e fenolada (0,45% de fenol – conservante), controle positivo (histamina) e o extrato de pulga total, todos em duplicata. Esses foram inoculados na derme com o auxílio de um puntor Duotip-Test II. As leituras foram realizadas após 15 minutos e sendo consideradas reações positivas aquelas pápulas que apresentaram diâmetro ortogonal médio igual ou superior a 3 mm da média do diâmetro do controle negativo. Foi feito também a leitura 24 e 48 horas depois para avaliar reações tardias. As reações observadas ao extrato das pulgas nos dois grupos foram fracas e consideradas como não reagentes. Os resultados encontrados indicam que o prick test utilizado nesse estudo não foi capaz de auxiliar no diagnóstico da DAPP. Sendo assim, mais estudos com pacientes atendidos na rotina clínica e com outros extratos são necessários para que se possa avaliar melhor a acurácia da técnica.por
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpor
dc.formatapplication/pdf*
dc.languageporpor
dc.publisherUniversidade Federal Rural do Rio de Janeiropor
dc.rightsAcesso Abertopor
dc.subjectalergiapor
dc.subjectcaninospor
dc.subjectpercutâneopor
dc.subjectpuntorpor
dc.subjectallergyeng
dc.subjectcanineseng
dc.subjectpercutaneouseng
dc.titleAvaliação do prick test como um método auxiliar de diagnóstico de dermatite alérgica a picada de pulga em cãespor
dc.title.alternativeEvaluation of the prick test as an auxiliary diagnostic method for flea allergy dermatites in dogseng
dc.typeTesepor
dc.description.abstractOtherFlea allergy dermatitis (FAD) is a common dermatological disease in Brazil, which brings discomfort for the animal and for the guardian. The diagnosis is usually based on anamnesis, physical examination and response to the treatment. These methods are questioned by the owner, now more enlightened and demanding, who wants a conclusive, objective and quick diagnosis. Some studies were made with the intradermal test (ID) to help on the disease diagnosis, but some results had divergencies. An alternative to help the diagnosis would be the use of prick tests (PT), which have been widely accepted in human medicine for being fast, safe, reliable and a low-cost method. The present study aimed to evaluate PT as an auxiliary tool in the diagnosis of FAD. 24 beagle dogs from the Laboratório de Quimioterapia Experimental em Parasitologia Veterinária (LQEPV), male and female, aged one year or more were selected. These animals were divided into two groups: group 1 containing 12 beagles known to have no FAD and group 2, composed of 12 dogs with clinical diagnosis of FAD. The animals were physically restrained, placed in lateral decubitus and had the chest area trichotomized and cleaned. One drop of each solution was used: for the negative control, a glycerin saline solution (50% glycerol - protein stabilizer) and phenolate (0.45% phenol - preservative), positive control (histamine) and the whole body flea extract, all in duplicate. There were inoculated into the dermis with the aid of a Duotip-Test II puntor. The readings were taken after 15 minutes and those papules that had mean orthogonal diameter equal to or greater than 3 mm of the mean diameter of the negative control were considered positive reactions. Reading 24 and 48 later was also done to assess delayed reactions. The reactions observed to the flea extract in both groups were weak and considered to be non-reactive. The results found indicate that the prick test used in this study was not able to assist in the diagnosis of FAD. Therefore, further studies with patients seen in the clinical routine and with other extracts are necessary in order to better assess the accuracy of the technique.eng
dc.contributor.advisor1Fernandes, Julio Israel
dc.contributor.advisor1ID082.100.187-60por
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/9221592908532393por
dc.contributor.advisor-co1Cunha, Victor do Espírito Santo
dc.contributor.advisor-co1ID042.425.127-24por
dc.contributor.referee1Fernandes, Julio Israel
dc.contributor.referee2Balthazar, Daniel de Almeida
dc.contributor.referee3dutra, Ary Elias Aboud
dc.creator.ID039.104.635-76por
dc.creator.Latteshttp://lattes.cnpq.br/0395549861537799por
dc.publisher.countryBrasilpor
dc.publisher.departmentInstituto de Veterináriapor
dc.publisher.initialsUFRRJpor
dc.publisher.programPrograma de Pós-Graduação em Ciências Veterináriaspor
dc.relation.referencesADKINSON JR, N. F. et al. Middleton’s allergy E-Book: Principles and practice. [s.l.] Elsevier Health Sciences, 2013. AMARANTE, C. F. do. Análise epidemiológica das dermatopatias de uma população canina atendida no período de 2005 a 2010 no Setor de Dermatologia do Hospital Veterinário da Universidade Federal Rural do Rio de Janeiro. 2012. Tese (Mestrado em Medicina Veterinária) - Faculdade de Medicina Veterinária, Univerdidade Federal Rural do Rio de Janeiro. Disponível em: < https://tede.ufrrj.br/jspui/handle/jspui/1780> BECSKEI, C. et al. Efficacy and safety of a novel oral isoxazoline, sarolaner (SimparicaTM) in the treatment of naturally occurring flea and tick infestations in dogs presented as veterinary patients in Europe. Veterinary Parasitology, v. 222, p. 49–55, 2016. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0304401716300322>. BECSKEI, C. et al. Efficacy of a novel oral chewable tablet containing sarolaner, moxidectin and pyrantel (Simparica TrioTM) against natural flea and tick infestations on dogs presented as veterinary patients in Europe. Parasites & Vectors, v. 13, n. 1, p. 72, 2020. Disponível em: Figura 8: Demarcação das pápulas com caneta dermográfica 15 minutos após a inoculação do controle negativo (A), controle positivo (B) e extrato do corpo de pulgas (C). <https://doi.org/10.1186/s13071-020-3946-1>. BERNSTEIN, I. L.; STORMS, W. W. Practice parameters for allergy diagnostic testing. Joint Task Force on Practice Parameters for the Diagnosis and Treatment of Asthma. The American Academy of Allergy, Asthma and Immunology and the American College of Allergy, Asthma and Immunology. Annals of allergy, asthma & immunology: official publication of the American College of Allergy, Asthma, & Immunology, v. 75, n. 6 Pt 2, p. 543, 1995. BONNEAU, S. et al. Therapeutic efficacy of topical hydrocortisone aceponate in experimental flea-allergy dermatitis in dogs. Australian Veterinary Journal, v. 87, n. 7, p. 287–291, 2009. BRUET, V. et al. Characterization of pruritus in canine atopic dermatitis, flea bite hypersensitivity and flea infestation and its role in diagnosis. Veterinary Dermatology, v. 23, n. 6, p. 1–8, 2012. CARDOSO, M. J. L. et al. Dermatopatias em cães: Revisão de 257 casos. Archives of Veterinary Science, v. 16, n. 2, p. 66–74, 2011. CARLOTTI, D. N.; COSTARGENT, F. Analysis of positive skin tests in 449 dogs with allergic dermatitis. European journal of companion animal practice, v. 4, p. 42–59, 1994. CARLOTTI, D. N.; JACOBS, D. E. Therapy, control and prevention of flea allergy dermatitis in dogs and cats. Veterinary Dermatology, v. 11, n. 2, p. 83–98, 2000. CARMONA-GIL, A. M.; SÁNCHEZ, J.; MALDONADO-ESTRADA, J. Evaluation of Skin Prick-Test Reactions for Allergic Sensitization in Dogs With Clinical Symptoms Compatible With Atopic Dermatitis. A Pilot Study. Frontiers in Veterinary Science, v. 6, n. December, p. 1–8, 2019. CAVALLERI, D. et al. A randomised, blinded, controlled field study to assess the efficacy and safety of lotilaner tablets (CredelioTM) in controlling fleas in client-owned dogs in European countries. Parasites & Vectors, v. 10, n. 1, p. 526, 2017. Disponível em: <https://doi.org/10.1186/s13071-017-2479-8>. CROSAZ, O. et al. Open field study on the efficacy of oral fluralaner for long-term control of flea allergy dermatitis in client-owned dogs in Ile-de-France region. Parasites & vectors, v. 9, n. 1, p. 1–5, 2016. DEBOER, D. J.; HILLIER, A. The ACVD task force on canine atopic dermatitis (XV): Fundamental concepts in clinical diagnosis. Veterinary Immunology and Immunopathology, v. 81, n. 3–4, p. 271–276, 20 set. 2001. DEMOLY, P. et al. Middleton’s allergy: principles and practice. 2009. DIAZ, S.; RADIN, M. J. Inflammatory Diseases of the SkinVeterinary Cytology: Wiley Online Books., 1 set. 2020. . Disponível em: <https://doi.org/10.1002/9781119380559.ch11>. DICKIN, S. K. et al. Efficacy of selamectin in the treatment and control of clinical signs of flea allergy dermatitis in dogs and cats experimentally infested with fleas. Journal of the American Veterinary Medical Association, v. 223, n. 5, p. 639–644, 2003. EAACI, D. S. et al. Position paper: allergen standardization and skin tests. The European Academy of Allergology and Clinical Immunology. Allergy, v. 48, n. 14 Suppl, p. 48–82, 1993. FISARA, P. et al. An open, self-controlled study on the efficacy of topical indoxacarb for eliminating fleas and clinical signs of flea-allergy dermatitis in client-owned dogs in Queensland, Australia. Veterinary Dermatology, v. 25, n. 3, p. 195–199, 2014. FOSTER, A. P. Flea allergy dermatitis in the dog. UK Vet Companion Animal, v. 11, n. 8, p. 63–66, 2006. FOSTER, A. P.; FOIL, C. S. BSAVA manual of small animal dermatology. [s.l: s.n.] GAMITO, M. S. R. Dermatites parasitárias no cãoUniversidade Técnica de Lisboa. Faculdade de Medicina Veterinária, , 2009. . GASPARETTO, N. D. et al. Prevalência das doenças de pele não neoplásicas em cães no município de Cuiabá, Mato Grosso. Pesquisa Veterinária Brasileira, v. 33, n. 3, p. 359–362, 2013. GREENE, W. K. et al. Characterization of allergens of the cat flea, Ctenocephalides felis: detection and frequency of IgE antibodies in canine sera. Parasite Immunology, v. 15, n. 2, p. 69–74, 1993. GROSS, T. L. et al. Doenças de pele do cão e do gato: diagnóstico clínico e histopatológico. Roca, São Paulo. 889p, 2009. HALLIWELL, R. E.; PRESTON, J. F.; NESBITT, J. G. Aspects of the immunopathogenesis of flea allergy dermatitis in dogs. Veterinary immunology and immunopathology, v. 17, n. 1–4, p. 483–494, 1987. HALLIWELL, R. E. W.; LONGINO, S. J. IgE and IgG antibodies to flea antigen in differing dog populations. Veterinary Immunology and Immunopathology, v. 8, n. 3, p. 215–223, 1985. HEINRICH, N. A. et al. Skin Diseases of the Dog and Cat. [s.l.] CRC Press, 2018. HENSEL, P. et al. Determination of threshold concentrations of allergens and evaluation of two different histamine concentrations in canine intradermal testing. Veterinary dermatology, v. 15, n. 5, p. 304–308, 2004. HILL, P. B. Small animal dermatology: A practical guide to the diagnosis and management of skin diseases in dogs and cats. [s.l.] Elsevier Health Sciences, 2002. HNILICA, K. A.; PATTERSON, A. P. Small Animal Dermatology-E-Book: A Color Atlas and Therapeutic Guide. [s.l.] Elsevier Health Sciences, 2016. KING, M. J.; LOCKEY, R. F. Allergen prick-puncture skin testing in the elderly. Drugs & aging, v. 20, n. 14, p. 1011–1017, 2003. LAFFORT-DASSOT, C. et al. Diagnosis of flea allergy dermatitis: Comparison of intradermal testing with flea allergens and a FcεRI α-based IgE assay in response to flea control. Veterinary Dermatology, v. 15, n. 5, p. 321–330, 2004. LAFFORT-DASSOT, C. Flea allergy in dogs: clinical signs and diagnosis. European Journal of Companion Animal Practice, v. 19, n. 3, p. 242–248, 2009. Disponível em: <http://www.fecava.org/files/EJCAP 19-3 p 242_248_Laffort.pdf>. LAM, A.; YU, A. Overview of flea allergy dermatitis. Compendium (Yardley, PA), v. 31, n. 5, p. E1-10, 2009. LEE, S. E. et al. Putative salivary allergens of the cat flea, Ctenocephalides felis felis. Veterinary immunology and immunopathology, v. 69, n. 2–4, p. 229–237, 1999. LEE, S. E.; JACKSON, L. A.; OPDEBEECK, J. P. Salivary antigens of the cat flea, Ctenocephalides felis felis. Parasite immunology, v. 19, n. 1, p. 13–19, 1997. LEUNG, D. Y. M. Atopic dermatitis: the skin as a window into the pathogenesis of chronic allergic diseases. Journal of allergy and clinical immunology, v. 96, n. 3, p. 302–319, 1995. MACHADO, G. A. C. Dermatopatias diagnosticadas em cães no hospital de medicina veterinária da Universidade Federal da Bahia por avaliações histopatológicas (2007-2016) e clínico-laboratoriais (2015-2017). 2017. Tese (Mestrado em Medicina Veterinária) - Escola de Medicina Veterinária e Zootecnia, Universidade Federal da Bahia. Disponível em: < http://repositorio.ufba.br/ri/handle/ri/31758> MCDERMOTT, M. J. et al. Identification, cloning, and characterization of a major cat flea salivary allergen (Cte f 1). Molecular Immunology, v. 37, n. 7, p. 361–375, 2000. MILLER JR, W. H.; GRIFFIN, C. E.; CAMPBELL, K. L. Muller and Kirk’s small animal dermatology. [s.l.] Elsevier Health Sciences, 2012. PATEL, A.; FORSYTHE, P. Dermatite alérgica à picada de pulgas. ______. Dermatologia em pequenos animais. 1ed. Rio de Janeiro: Elsevier, p. 28–34, 2010. PUCHEU-HASTON, C. M. et al. Allergenic cross-reactivities in flea-reactive canine serum samples. American journal of veterinary research, v. 57, n. 7, p. 1000–1005, 1996. REEDY, L. M.; MILLER, W. H.; WILLEMSE, T. Introduction to allergy. Allergic Skin Diseases of Dogs and Cats. 2nd ed. London, UK: WB Saunders, p. 1–24, 1999. RHODES, K. H.; WERNER, A. H. Blackwell’s five-minute veterinary consult clinical companion: small animal dermatology. [s.l.] John Wiley & Sons, 2018. ROHDICH, N.; ROEPKE, R. K. A.; ZSCHIESCHE, E. A randomized, blinded, controlled and multi-centered field study comparing the efficacy and safety of BravectoTM (fluralaner) against FrontlineTM (fipronil) in flea- and tick-infested dogs. Parasites & Vectors, v. 7, n. 1, p. 83, 2014. Disponível em: <https://doi.org/10.1186/1756-3305-7-83>. ROSSI, M. A. et al. A pilot study of the validation of percutaneous testing in cats. Veterinary dermatology, v. 24, n. 5, p. 488-e115, 2013. SALZO, P. S. Dermatite atópica. Tratado de Medicina Externa-Dermatologia Veterinária. São Caetano do Sul: Interbook, p. 513–529, 2016. SCOTT, D. W.; MILLER, D. H.; GRIFFIN, C. E. Muller and Kirk’s Small Animal. Dermatology, 6th edn. WB Saunders, Philadelphia, 2001. SISCHO, W. M.; IHRKE, P. J.; FRANTI, C. E. Regional distribution of ten common skin diseases in dogs. Journal of the American Veterinary Medical Association, v. 195, n. 6, p. 752–756, 1989. SOUZA, T. M. et al. Prevalência das dermatopatias não-tumorais em cães do município de Santa Maria, Rio Grande do Sul (2005-2008) Pesquisa Veterinária Brasileira scielo , , 2009. STOLPER, R.; OPDEBEECK, J. P. Flea allergy dermatitis in dogs diagnosed by intradermal skin tests. Research in veterinary science, v. 57, n. 1, p. 21–27, 1994. TIZARD, I. R. Veterinary Immunology-E-Book. [s.l.] Elsevier Health Sciences, 2017. VON RUEDORFFER, U. et al. Flea Bite Hypersensitivity: New Aspects On The Involvement Of Mast Cells. The Veterinary Journal, v. 165, n. 2, p. 149–156, 2003. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1090023302001600>.por
dc.subject.cnpqMedicina Veterináriapor
dc.thumbnail.urlhttps://tede.ufrrj.br/retrieve/73090/2021%20-%20Lais%20de%20Jesus%20Moraes.pdf.jpg*
dc.originais.urihttps://tede.ufrrj.br/jspui/handle/jspui/6556
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