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DC Field | Value | Language |
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dc.contributor.author | Farias, Alan Marques | |
dc.date.accessioned | 2023-12-22T01:46:10Z | - |
dc.date.available | 2023-12-22T01:46:10Z | - |
dc.date.issued | 2022-10-20 | |
dc.identifier.citation | FARIAS, Alan Marques. Microencapsulação de β-caroteno pela coacervação complexa empregando como material de parede ovalbumina e alginato de sódio. 2022. 75 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2022. | por |
dc.identifier.uri | https://rima.ufrrj.br/jspui/handle/20.500.14407/11059 | - |
dc.description.abstract | O β-caroteno (βC) é um composto lipossolúvel natural necessário para a saúde humana, sendo uma fonte alimentar importante de provitamina A, mas apresenta alta instabilidade química, que aumenta sua oxidação na presença de fatores extrínsecos. O presente estudo teve como objetivo microencapular βC em complexos coacervados formados pela interação entre ovalbumina (OVA) e alginato de sódio (NaAlg). A técnica de microencapsulação foi empregada em pH 4,0 e proporção 8:1 (OVA:NaAlg, m/m) após confirmação da afinidade desses complexos com potencial zeta, estudo do diagrama de fases, análises turbidimétrica e de calorimetria de titulação isotérmica. As cápsulas formadas a partir do complexo OVA:NaAlg apresentaram morfologia esférica com núcleo bem definido e alta eficiência de encapsulação (~ 97,5 %). A presença de OVA, NaAlg e βC nas microcápsulas foram confirmadas pela análise de espectroscopia no infravermelho. A Simulação da digestão gastrointestinal in vitro das microcápsulas constatou que 71,39 % do βC encapsulado foi liberado no intestino, com bioacessibilidade de 32,78 %. O perfil cinético de liberação se ajustou melhor ao modelo de primeira ordem (R² = 0,98), cujo mecanismo de liberação é por difusão, de modo que o βC encapsulado migra do meio mais concentrado para o menos concentrado por meio de poros formados no material de parede. Atividade antioxidante foi comprovada ao aplicar microcápsulas de βC na produção de biscoitos, resultando no dobro de proteção do bioativo em relação ao βC livre. Assim, os resultados apresentados sugerem que as microcápsulas de βC formadas com os material de parede OVA:NaAlg podem ser inseridas eficientemente na fortificação de biscoitos. | por |
dc.description.sponsorship | CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior | por |
dc.format | application/pdf | * |
dc.language | por | por |
dc.publisher | Universidade Federal Rural do Rio de Janeiro | por |
dc.rights | Acesso Aberto | por |
dc.subject | Bioacessibilidade | por |
dc.subject | β-caroteno encapsulado | por |
dc.subject | Simulação gastrointestinal | por |
dc.subject | Atividade antioxidante | por |
dc.subject | Bioaccessibility | eng |
dc.subject | encapsulated β-carotene | eng |
dc.subject | Gastrointestinal simulation | eng |
dc.subject | Antioxidant activity | eng |
dc.title | Microencapsulação de β-caroteno pela coacervação complexa empregando como material de parede ovalbumina e alginato de sódio | por |
dc.title.alternative | Microencapsulation of β-carotene by complex coacervation using ovalbumin and sodium alginate as wall material | eng |
dc.type | Dissertação | por |
dc.description.abstractOther | β-carotene (βC) is a natural fat-soluble compound necessary for human health, being an important food source of provitamin A, but it has high chemical instability, which increases its oxidation in the presence of extrinsic factors. The present study aimed to microencaulate βC in complex coacervated formed by the interaction between ovalbumin (OVA) and sodium alginate (NaAlg). The microencapsulation technique was used at pH 4.0 and 8:1 ratio (OVA:NaAlg, w/w) after confirming the affinity of these complexes with zeta potential, study of the phase diagram, turbidimetric and isothermal titration calorimetry analyses. The capsules formed from the OVA:NaAlg complex showed spherical morphology with a well-defined nucleus and high encapsulation efficiency (~97.5 %). The presence of OVA, NaAlg and βC in the microcapsules were confirmed by Fourier Transform Infrared Spectroscopy (FTIR). The in vitro gastrointestinal digestion simulation of microcapsules found that 71.39 % of the encapsulated βC was released in the intestine, with a bioaccessibility of 32.78 %. The kinetic release profile was better adjusted to the first order model (R² = 0.98), whose release mechanism is by diffusion, so that the encapsulated βC migrates from the more concentrated medium to the less concentrated one through pores formed in the wall material. Antioxidant activity was proven when applying βC microcapsules in the production of cookies, resulting in twice the protection of the bioactive in relation to free βC. Thus, the results presented suggest that βC microcapsules formed with the OVA:NaAlg wall material can be efficiently inserted in biscuit fortification. | eng |
dc.contributor.advisor1 | Garcia-Rojas, Edwin Elard | |
dc.contributor.advisor1ID | 014.548.996-54 | por |
dc.contributor.advisor1Lattes | http://lattes.cnpq.br/1205756654416987 | por |
dc.contributor.referee1 | Garcia-Rojas, Edwin Elard | |
dc.contributor.referee2 | Costa, Bernardo de Sá | |
dc.contributor.referee3 | Vicente, Juarez | |
dc.creator.ID | 114.400.647-33 | por |
dc.creator.Lattes | http://lattes.cnpq.br/9632732420242567 | por |
dc.publisher.country | Brasil | por |
dc.publisher.department | Instituto de Tecnologia | por |
dc.publisher.initials | UFRRJ | por |
dc.publisher.program | Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos | por |
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May, p. 105978, 2020. | por |
dc.subject.cnpq | Ciência e Tecnologia de Alimentos | por |
dc.thumbnail.url | https://tede.ufrrj.br/retrieve/73474/2022%20-%20Alan%20Marques%20Farias.Pdf.jpg | * |
dc.originais.uri | https://tede.ufrrj.br/jspui/handle/jspui/6649 | |
dc.originais.provenance | Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2023-05-30T19:49:14Z No. of bitstreams: 1 2022 - Alan Marques Farias.Pdf: 2573993 bytes, checksum: 70865ca937274dc25d9a387585886ed5 (MD5) | eng |
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Appears in Collections: | Mestrado em Ciência e Tecnologia de Alimentos |
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