Please use this identifier to cite or link to this item: https://rima.ufrrj.br/jspui/handle/20.500.14407/16048
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dc.contributor.authorRosa, Maurilio-
dc.date.accessioned2024-03-05T13:34:08Z-
dc.date.available2024-03-05T13:34:08Z-
dc.date.issued2022-06-10-
dc.identifier.citationROSA, Maurílio. Caracterização morfológica de biomateriais utilizando a membrana amniótica equina como substrato. 2022. 81 f. Tese (Doutorado em Medicina Veterinária) - Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica. 2022.pt_BR
dc.identifier.urihttps://rima.ufrrj.br/jspui/handle/20.500.14407/16048-
dc.description.abstractNeste trabalho, buscou-se estabelecer e validar protocolos para coleta, preparo e preservação da membrana amniótica equina que possibilitem a obtenção de um biomaterial viável e livre de contaminação para utilização com fins terapêuticos. Foram testados e estudados os processos de preparo de três diferentes tipos de biomateriais, a membrana restituída de congelamento (AMNn), a membrana desidratada (AMNd) e a membrana descelularizada (AMNTx). Cada um desses materiais também foi caracterizado morfologicamente por imunofluorescência. Complementando o estudo, foi realizada a avaliação clínica pela utilização de cada biomaterial através do tratamento de reparo de lesões corneais em queratomalacia. O resultado do estudo dos processos de coleta, preparo e preservação, demonstrou ser eficiente para produção dos biomateriais. Os materiais AMNTx e AMNn foram os que demandaram mais recursos na sua produção e métodos de armazenamento. O biomaterial AMNd foi o que despendeu menores recursos de produção e armazenamento, demonstrando ser um material versátil capaz de ser utilizado em locais com pouca infraestrutura. Os resultados da caracterização morfológica por imunofluorescência de cada biomaterial culminaram com a comprovação da manutenção dos componentes laminina, fibronectina e dos colágenos I e IV na estrutura da maioria das amostras dos biomateriais estudados, com exceção da AMNd para colágeno I, que apresentou resultado inconclusivo. A avaliação dos tratamentos mostrou que a utilização desses biomateriais foi capaz de reduzir a formação de tecido cicatricial nas lesões. A comparação desses resultados também demonstrou que a membrana desidratada obteve o melhor desempenho clínico perante as membranas congeladas e descelurarizadas, quando observadas a quantidade de formação de tecido cicatricial, vascularização e área de córnea clara preservada. Essas informações obtidas tornam-se de suma importância, uma vez que estes biomateriais demonstraram que podem ser utilizados como bandagens curativas, servindo como arcabouço para migração, adesão, multiplicação celular, estímulo à angiogênese e controle da inflamação, durante o processo de cicatrização tecidual.pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESpt_BR
dc.languageporpt_BR
dc.publisherUniversidade Federal Rural do Rio de Janeiropt_BR
dc.subjectBiomateriaispt_BR
dc.subjectMembrana amnióticapt_BR
dc.subjectEquinospt_BR
dc.subjectBiomaterialspt_BR
dc.subjectAmniotic membranept_BR
dc.subjectEquinept_BR
dc.titleImunomarcação dos componentes da matriz extracelular da membrana amniótica equina sob diferentes métodos de conservação e sua aplicação clínicapt_BR
dc.title.alternativeMorphological characterization of biomaterials using the equine amniotic membrane as substrate.en
dc.typeTesept_BR
dc.description.abstractOtherIn this work, we sought to establish and validate protocols for the collection, preparation and preservation of the equine amniotic membrane that make it possible to obtain a viable and contamination-free biomaterial for use with therapeutic purposes. The processes of preparation of three different types of biomaterials were tested and studied, the frozen-restituted membrane (AMNn), the dehydrated membrane (AMNd) and the decellularized membrane (AMNTx). Each of these materials was also morphologically characterized by immunofluorescence. Complementing the study, a clinical evaluation was performed using each biomaterial in the repair treatment of corneal lesions in keratomalacia. The result of the study of the collection, preparation and preservation processes proved to be efficient to produce biomaterials. AMNTx and AMNn were materials that demanded more resources in their production and storage methods. The AMNd biomaterial required less production and storage resources, proving to be a versatile material capable of being used in places with little infrastructure. The results of the morphological characterization by immunofluorescence of each biomaterial resulted in proof of the maintenance of the components laminin, fibronectin, and collagens I and IV in the structure of most samples of the studied biomaterials, except for AMNd for collagen I, which showed inconclusive results. The evaluation of the treatments showed that the use of these biomaterials was able to reduce the scar tissue formation in the lesions. The comparison of these results also showed that the dehydrated membrane had the best clinical performance against the frozen and decellularized membranes, when observing the amount of scar tissue formation, vascularization and preserved clear corneal area. This information obtained becomes of paramount importance, since these biomaterials have demonstrated that they can be used as curative bandages, serving as a scaffold for migration, adhesion, cell multiplication, stimulation of angiogenesis and control of inflammation during the tissue healing process.en
dc.contributor.advisor1Botteon, Paulo de Tarso Landgraf-
dc.contributor.advisor1IDhttps://orcid.org/0000-0002-9695-4217pt_BR
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/6104411705808124pt_BR
dc.contributor.referee1Botteon, Paulo de Tarso Landgraf-
dc.contributor.referee1IDhttps://orcid.org/0000-0002-9695-4217pt_BR
dc.contributor.referee1Latteshttp://lattes.cnpq.br/6104411705808124pt_BR
dc.contributor.referee2Silva, Marta Fernanda Albuquerque da-
dc.contributor.referee2Latteshttp://lattes.cnpq.br/0544591266946007pt_BR
dc.contributor.referee3Carvalho, Vivian de Assunção Nogueira-
dc.contributor.referee3IDhttps://orcid.org/0000-0002-4034-9675pt_BR
dc.contributor.referee3Latteshttp://lattes.cnpq.br/6012855368365110pt_BR
dc.contributor.referee4Andrade, Alexandre Lima de-
dc.contributor.referee4IDhttps://orcid.org/0000-0002-3462-4722pt_BR
dc.contributor.referee4Latteshttp://lattes.cnpq.br/6404176495306171pt_BR
dc.contributor.referee5Pellizzon, Claudia Helena-
dc.contributor.referee5Lattesttp://lattes.cnpq.br/0019393779801069pt_BR
dc.creator.ID509.514.837-49pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/2803066160311519pt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentInstituto de Veterináriapt_BR
dc.publisher.initialsUFRRJpt_BR
dc.publisher.programPrograma de Pós-Graduação em Medicina Veterinária (Patologia e Ciências Clínicas)pt_BR
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Current Diabetes Reports, v.20, n.8, 31, p.1-10, 2020. WELCH, P.M.; GABAL, M.; BETTS, D.M.; WHELAN, N.C.; STUDER, M.E. In vitro analysis of antiangiogenic activity of fungi isolated from clinical cases of equine keratomycosis. Journal of Veterinary Ophthalmology, v.3, p.145-151, 2000.pt_BR
dc.subject.cnpqGenéticapt_BR
dc.subject.cnpqMedicina Veterináriapt_BR
dc.subject.cnpqZoologiapt_BR
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